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Sample GSM4981054 Query DataSets for GSM4981054
Status Public on Dec 19, 2020
Title deep senescent cells - H3K27me3_rep1
Sample type SRA
Source name foreskin fibroblast
Organism Homo sapiens
Characteristics cell line: BJ
treatment: bleomycin (40 ug/ml; Biorbyt) for 2 hours and culture for 28 days
cell state: DS
chip antibody: H3K27me3
Extracted molecule genomic DNA
Extraction protocol ChIP experiment was carried out as previously described (Hawkins et al., 2010) with H3K9me3 (Abcam, ab8898) and H3K27m3(CST, 9733s) antibodies.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NovaSeq 6000
Data processing mapping to hg19 with bowtie2
deduplication by Picard
macs2 callpeak --SPMR, and get pileup files
Genome_build: hg19
Supplementary_files_format_and_content: bigwig, normalized pileup files
Submission date Dec 18, 2020
Last update date Dec 22, 2020
Contact name Xianglin Zhang
Phone 15101669184
Organization name Tsinghua University
Street address Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
Platform ID GPL24676
Series (1)
GSE133292 The loss of heterochromatin is associated with multiscale three-dimensional genome reorganization and aberrant transcription during cellular senescence
BioSample SAMN17117889
SRA SRX9704400

Supplementary file Size Download File type/resource 158.5 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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