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Sample GSM4981046 Query DataSets for GSM4981046
Status Public on Dec 19, 2020
Title deep senescent cells - H3K9me3_rep1
Sample type SRA
 
Source name foreskin fibroblast
Organism Homo sapiens
Characteristics cell line: BJ
treatment: bleomycin (40 ug/ml; Biorbyt) for 2 hours and culture for 28 days
cell state: DS
chip antibody: H3K9me3
Extracted molecule genomic DNA
Extraction protocol ChIP experiment was carried out as previously described (Hawkins et al., 2010) with H3K9me3 (Abcam, ab8898) and H3K27m3(CST, 9733s) antibodies.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NovaSeq 6000
 
Data processing mapping to hg19 with bowtie2
deduplication by Picard
macs2 callpeak --SPMR, and get pileup files
Genome_build: hg19
Supplementary_files_format_and_content: bigwig, normalized pileup files
 
Submission date Dec 18, 2020
Last update date Dec 22, 2020
Contact name Xianglin Zhang
E-mail(s) xl-zhang15@mails.tsinghua.edu.cn
Phone 15101669184
Organization name Tsinghua University
Street address Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100084
Country China
 
Platform ID GPL24676
Series (1)
GSE133292 The loss of heterochromatin is associated with multiscale three-dimensional genome reorganization and aberrant transcription during cellular senescence
Relations
BioSample SAMN17117897
SRA SRX9704392

Supplementary file Size Download File type/resource
GSM4981046_DS1_H3K9me3_pileup.bw 131.9 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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