|
| Status |
Public on Sep 11, 2023 |
| Title |
HN2 C5 shNTC 1 |
| Sample type |
RNA |
| |
|
| Source name |
mesenchymal chondrosarcoma cell introduced with shRNA of non-target control
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mesenchymal chondrosarcoma cell
treatment: introduced with shRNA of non-target control
|
| Treatment protocol |
Short hairpin RNA-mediated gene silencing for HEY1-NCOA2 or Runx2 was performed
|
| Growth protocol |
Sarcoma cells and chondrogenic progenitor cells were maintained in IMDM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
|
| Description |
Gene expression data from mouse mesenchymal chondrosarcoma
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 16, 2020 |
| Last update date |
Sep 11, 2023 |
| Contact name |
Takuro Nakamura |
| E-mail(s) |
takuro-ind@umin.net
|
| Phone |
81-3-3570-0462
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
The Cancer Institute
|
| Lab |
Carcinogenesis
|
| Street address |
3-8-31 Ariake, Koto-ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE163291 |
HEY1-NCOA2 interacts with RUNX2 to induce mesenchymal chondrosarcoma [Array] |
| GSE163588 |
HEY1-NCOA2 interacts with RUNX2 to induce mesenchymal chondrosarcoma |
|
