|
| Status |
Public on Jul 01, 2021 |
| Title |
MA-10 Leydig cells transfected with siRNA targeting COUP-TFII, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
MA-10 Leydig cells transfected with siRNA targeting COUP-TFII
|
| Organism |
Mus musculus |
| Characteristics |
cell line: MA-10 Leydig cells
|
| Treatment protocol |
Cells were transiently transfected with siRNAs as indicated above for 48 hours.
|
| Growth protocol |
MA-10 Leydig cells were grown in DMEM/F12 medium supplemented with 2.438 g/L sodium bicarbonate, 3.57 g/L HEPES, 15% horse serum, 50 mg/L penicillin and streptomycin sulphate on plates coated with 0.1% gelatin at 37 Celsius (C) and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA mixes were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
|
| |
|
| Hybridization protocol |
The labeled cDNA mixes were hybridized for 16 hr at 45C on Affymetrix Mouse Gene 1.0 ST arrays. GeneChips were washed and stained in the Affymetrix GeneChip fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip™ Scanner 3000 7G.
|
| Description |
gene expression data from siRNA COUP-TFII transfected cells
|
| Data processing |
The data was analyzed using Partek genomics suite 7.0 (Partek, Inc. St. Louis, MO, USA) according to the manufacturer’s instructions.
|
| |
|
| Submission date |
Dec 15, 2020 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Jacques J. Tremblay |
| E-mail(s) |
jacques-j.tremblay@crchudequebec.ulaval.ca
|
| Organization name |
Centre de recherche du CHU de Québec
|
| Department |
Reproduction, Mother and Child Health
|
| Street address |
2705 Laurier Blvd.
|
| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE163283 |
Expression data from NR2F2(COUP-TFII) depleted mouse MA-10 Leydig cells |
|
