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Sample GSM497456 Query DataSets for GSM497456
Status Public on Jan 16, 2010
Title Liver rosiglitazone 28µmol/kg technical replicate 4
Sample type RNA
 
Channel 1
Source name Pool of db/db control mice
Organism Mus musculus
Characteristics tissue: Liver
strain: BKS Cg-m+/+ Leprdb/J
Treatment protocol Per os treatment for 18 days
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3,Cy5
Label protocol Total RNA samples were labelled using Agilent low RNA input linear amplification Kit, according to the manufacturer's protocols. 500ng of liver and IAT total RNA were reversed transcribed by MMLV reverse transcriptase. Amplification and labelling were done by T7-polymerase transcription. Test and reference cRNA were labelled with cyanine Cy5 or Cy3-CTP dyes.
 
Channel 2
Source name db/db mouse treated with 28µmol/kg of rosiglitazone
Organism Mus musculus
Characteristics tissue: Liver
strain: BKS Cg-m+/+ Leprdb/J
Treatment protocol Per os treatment for 18 days
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5,Cy3
Label protocol Total RNA samples were labelled using Agilent low RNA input linear amplification Kit, according to the manufacturer's protocols. 500ng of liver and IAT total RNA were reversed transcribed by MMLV reverse transcriptase. Amplification and labelling were done by T7-polymerase transcription. Test and reference cRNA were labelled with cyanine Cy5 or Cy3-CTP dyes.
 
 
Hybridization protocol Samples were incubated at 60°C for 30 minutes and then hybridized for 17 hours at 65°C in a 10 rpm rotary oven. Slides were then washed in buffer 1 and 2, according to manufacturer’s specification.
Scan protocol Scanned on an Agilent G2505B scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.5).
Description Analysis used the pool of db/db mice as control samples for comparison to the treated mice. Technical replicate 4 of 6. Db/db mice treated with 28µmol/kg of rosiglitazone
Data processing Agilent Feature Extraction Software (v 9.5) was used for background subtraction and LOWESS normalization (GE2-v5_95_Feb07 protocol). Rosetta Resolver (v 7.1) was used for the dye-swap combination.
 
Submission date Jan 14, 2010
Last update date Jan 15, 2010
Contact name Aurélie Cotillard
Organization name Institut de Recherches Servier
Street address 125 chemin de ronde
City Croissy-sur-Seine
ZIP/Postal code 78290
Country France
 
Platform ID GPL7202
Series (1)
GSE19896 Diabetic db/db mice treated with three doses of rosiglitazone and S compound

Data table header descriptions
ID_REF
VALUE LOWESS normalized log ratio (experiment/control)

Data table
ID_REF VALUE
A_51_P362483 -0.171177
A_51_P199927 -0.006188
A_51_P353539 0
A_51_P386899 -0.017708
A_51_P428364 0.068546
A_51_P467268 0
A_51_P428360 0
A_51_P411345 -0.007567
A_51_P175871 0.071651
A_51_P373428 0.544964
A_51_P139641 0.001124
A_51_P373424 0.030901
A_51_P504902 -0.017116
A_51_P397834 -0.106248
A_51_P366811 -0.042137
A_51_P407304 -0.181438
A_51_P454707 -0.011012
A_51_P353502 -0.067029
A_51_P405496 0
A_51_P504503 0.021099

Total number of rows: 41174

Table truncated, full table size 845 Kbytes.




Supplementary file Size Download File type/resource
GSM497456_Liver_Rosi28_1_t4_Cy3_ref_Cy5_test.txt.gz 14.3 Mb (ftp)(http) TXT
GSM497456_Liver_Rosi28_1_t4_Cy3_test_Cy5_ref.txt.gz 14.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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