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Sample GSM497388 Query DataSets for GSM497388
Status Public on Jan 16, 2010
Title Liver rosiglitazone 84µmol/kg biological replicate 2
Sample type RNA
 
Channel 1
Source name Pool of db/db control mice
Organism Mus musculus
Characteristics tissue: Liver
strain: BKS Cg-m+/+ Leprdb/J
Treatment protocol Per os treatment for 18 days
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3,Cy5
Label protocol Total RNA samples were labelled using Agilent low RNA input linear amplification Kit, according to the manufacturer's protocols. 500ng of liver and IAT total RNA were reversed transcribed by MMLV reverse transcriptase. Amplification and labelling were done by T7-polymerase transcription. Test and reference cRNA were labelled with cyanine Cy5 or Cy3-CTP dyes.
 
Channel 2
Source name db/db mouse treated with 84µmol/kg of rosiglitazone
Organism Mus musculus
Characteristics tissue: Liver
strain: BKS Cg-m+/+ Leprdb/J
Treatment protocol Per os treatment for 18 days
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5,Cy3
Label protocol Total RNA samples were labelled using Agilent low RNA input linear amplification Kit, according to the manufacturer's protocols. 500ng of liver and IAT total RNA were reversed transcribed by MMLV reverse transcriptase. Amplification and labelling were done by T7-polymerase transcription. Test and reference cRNA were labelled with cyanine Cy5 or Cy3-CTP dyes.
 
 
Hybridization protocol Samples were incubated at 60°C for 30 minutes and then hybridized for 17 hours at 65°C in a 10 rpm rotary oven. Slides were then washed in buffer 1 and 2, according to manufacturer’s specification.
Scan protocol Scanned on an Agilent G2505B scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.5).
Description Analysis used the pool of db/db mice as control samples for comparison to the treated mice.Biological replicate 2 of 6. Db/db mice treated with 84µmol/kg of rosiglitazone
Data processing Agilent Feature Extraction Software (v 9.5) was used for background subtraction and LOWESS normalization (GE2-v5_95_Feb07 protocol). Rosetta Resolver (v 7.1) was used for the dye-swap combination.
 
Submission date Jan 14, 2010
Last update date Jan 15, 2010
Contact name Aurélie Cotillard
Organization name Institut de Recherches Servier
Street address 125 chemin de ronde
City Croissy-sur-Seine
ZIP/Postal code 78290
Country France
 
Platform ID GPL7202
Series (1)
GSE19896 Diabetic db/db mice treated with three doses of rosiglitazone and S compound

Data table header descriptions
ID_REF
VALUE LOWESS normalized log ratio (experiment/control)

Data table
ID_REF VALUE
A_51_P362483 -0.095776
A_51_P199927 -0.040433
A_51_P353539 0.061272
A_51_P386899 -0.073766
A_51_P428364 0.054903
A_51_P467268 0
A_51_P428360 0
A_51_P411345 0.013104
A_51_P175871 0.052483
A_51_P373428 0.403889
A_51_P139641 -0.047511
A_51_P373424 0
A_51_P504902 -0.063187
A_51_P397834 -0.101909
A_51_P366811 0.223348
A_51_P407304 -0.177699
A_51_P454707 -0.010425
A_51_P353502 -0.065163
A_51_P405496 0
A_51_P504503 -0.029347

Total number of rows: 41174

Table truncated, full table size 822 Kbytes.




Supplementary file Size Download File type/resource
GSM497388_Liver_Rosi84_2_Cy3_ref_Cy5_test.txt.gz 14.3 Mb (ftp)(http) TXT
GSM497388_Liver_Rosi84_2_Cy3_test_Cy5_ref.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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