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Sample GSM495835 Query DataSets for GSM495835
Status Public on Jan 13, 2010
Title Patient_48_6hr_AS602868_10µM_rep1
Sample type RNA
 
Source name Leukemic blasts, 6hr, AS602868, 10µM, repilicate 1
Organism Homo sapiens
Characteristics cell type: leukemic blast
gender: f
age: 71y
Treatment protocol Isolated leukemic blasts from AML patients were incubated in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum in presence or absence of 10 µM AS602868 for 6 hours at 37 ºC in a humidified incubator with 5% CO2
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations and quantified on a Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). RNA integrity was verified using the BioAnalyser 2100™ (Agilent Technologies, Palo Alto, CA, USA).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer
 
Hybridization protocol Cy3-labelled cRNA was fragmented at 60 °C for 30 minutes in a reaction volume of 55 µl containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 55 µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Whole Human Genome 4 x 44K microarrays (G4112F) for 17 hours at 65 °C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37 °C GE Wash buffer 2 (Agilent).
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) with default settings, and data extracted by Feature Extraction software, version 9.5.
Description Gene expression after 6h exposure of human leukemic blasts to 10 µM AS602868
Data processing Data were normalized using the quantile method
 
Submission date Jan 12, 2010
Last update date Jan 12, 2010
Contact name Lars Petter Jordheim
E-mail(s) lars.jordheim@recherche.univ-lyon1.fr
Organization name INSERM U590
Street address 8 Av Rockefeller
City Lyon
ZIP/Postal code 69008
Country France
 
Platform ID GPL4133
Series (1)
GSE19853 Gene expression profile of fresh human acute myeloid leukemia cells exposed ex vivo to AS602868

Data table header descriptions
ID_REF
VALUE Log2 transformed quantile normalized signal intensity

Data table
ID_REF VALUE
556 8.04077487507698
775 1.52725083211289
824 1.43406936284896
1408 1.61718253015392
1778 1.28660662734659
2154 1.80735936180287
2240 1.81768282056678
2370 1.36349321181143
2391 1.66112722614965
2911 1.82543609104672
3154 1.67586862873344
3270 1.89687812150656
3350 1.91829744760048
4064 1.57286954516453
4080 1.31859580269787
4481 1.73218475809884
4641 1.87147739369709
4686 1.72801372080122
6457 1.46819021661035
6480 1.73358036178612

Total number of rows: 45015

Table truncated, full table size 995 Kbytes.




Supplementary file Size Download File type/resource
GSM495835_48_AS602868.txt.gz 7.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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