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Sample GSM4952633 Query DataSets for GSM4952633
Status Public on Jan 01, 2023
Title mASPS_2_ASPSCR1
Sample type SRA
 
Source name mouse tumor
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: ASPSCR1-TFE3 mouse model
chip antibody: ASPSCR1
tissue: mouse tumor
Extracted molecule genomic DNA
Extraction protocol Nuclei isolated from snap frozen samples. Lysates were clarified from sonicated nuclei and TARGET DNA complexes were isolated with antibody.
Library was prepared using NEBnext ChIP-seq libraray prep reagent set
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina MiSeq
 
Data processing single-end 50bp sequencing on an Illumina MiSeq platform
ChIP-seq reads were aligned to the mm10 genome assembly using BWA-MEM (version 0.7.10-r789)
peaks were called using MACS2 with following settings: -B --SPMR --pvalue=1e-2 --mfold 15 100
Genome_build: mm10
Supplementary_files_format_and_content: bigwig files were generated using MACS2; Scores represent a normalized pileup signal
 
Submission date Dec 01, 2020
Last update date Jan 01, 2023
Contact name Jinxiu Li
Organization name China Agricutural University
Department College of biological sciences
Lab State key laboratories of Agrobiotechnology
Street address Yuanmingyuan west road 2#
City Beijing
State/province Beijing
ZIP/Postal code 100193
Country China
 
Platform ID GPL16417
Series (2)
GSE162476 ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [mASPS_ChIP, ASPL_VCP_Pol2]
GSE162609 ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP
Relations
BioSample SAMN16974628
SRA SRX9616470

Supplementary file Size Download File type/resource
GSM4952633_mASPS-2_ASPSCR1.wig.gz 383.4 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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