|
| Status |
Public on Jan 27, 2021 |
| Title |
bulk_P1_EMRA |
| Sample type |
SRA |
| |
|
| Source name |
Donor 1, Effector (RA+) CD8+ T cell
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Effector (RA+) CD8+ T cell
|
| Growth protocol |
Cells were thawed and rested overnight at 37C in RPMI + 10% FBS + 1% L-glut + 1% Pen/Strep media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
GSM4456493
|
| Data processing |
Sequencing reads were aligned to the hg38 reference genome using STAR 2.5.2a
Data was normalized using DESeq2
Genome_build: hg38
Supplementary_files_format_and_content: comma-separated text file include normalized read counts for each sample
|
| |
|
| Submission date |
Nov 24, 2020 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Marcus Buggert |
| E-mail(s) |
marcus.buggert@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Microbiology
|
| Street address |
C2:94; Karolinska Universitetssjukhuset Huddinge
|
| City |
Stockholm |
| State/province |
PA |
| ZIP/Postal code |
141 86 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE162097 |
The identity of human tissue-emigrant CD8+ T cells (scRNAseq, scTCRseq, bulk RNA-seq) |
| GSE162180 |
The identity of human tissue-emigrant CD8+ T cells |
|
| Relations |
| Alternative to |
GSM4456493 |
| BioSample |
SAMN16883166 |
| SRA |
SRX9568887 |
