|
| Status |
Public on Jan 05, 2010 |
| Title |
Identification of miR-489 target genes in HSC3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
control miRNA transfected HSC3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HSC3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Channel 2 |
| Source name |
miR-489 transfected HSC3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HSC3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software.
|
| Description |
miRNA treated in 72hr in head and neck cancer cell line HSC3
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Dec 31, 2009 |
| Last update date |
Jan 04, 2010 |
| Contact name |
Naohiko Seki |
| E-mail(s) |
naoseki@faculty.chiba-u.jp
|
| Phone |
+81-43-226-2971
|
| Organization name |
Chiba University
|
| Department |
Functional Genomics
|
| Street address |
1-8-1, Inohana
|
| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE19718 |
Identification of miR-489 or miR-504 target genes in head and neck cancer |
|
