NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM492576 Query DataSets for GSM492576
Status Public on Jan 05, 2010
Title Identification of miR-489 target genes in FaDu
Sample type RNA
 
Channel 1
Source name control miRNA transfected FaDu
Organism Homo sapiens
Characteristics cell line: FaDu
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
Channel 2
Source name miR-489 transfected FaDu
Organism Homo sapiens
Characteristics cell line: FaDu
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol
 
 
Hybridization protocol Agilent two-color gene expression hyb/wash protocol
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software.
Description miRNA treated in 72hr in head and neck cancer cell line FaDu
Data processing LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
 
Submission date Dec 31, 2009
Last update date Jan 04, 2010
Contact name Naohiko Seki
E-mail(s) naoseki@faculty.chiba-u.jp
Phone +81-43-226-2971
Organization name Chiba University
Department Functional Genomics
Street address 1-8-1, Inohana
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL4133
Series (1)
GSE19718 Identification of miR-489 or miR-504 target genes in head and neck cancer

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -4.107809548e-001
2 0.000000000e+000
3 0.000000000e+000
4 0.000000000e+000
5 -6.865043066e-001
6 0.000000000e+000
7 0.000000000e+000
8 0.000000000e+000
9 0.000000000e+000
10 0.000000000e+000
11 -3.020635231e-001
12 -3.078056716e-002
13 -6.448256106e-002
14 -1.074924038e-001
15 1.631013119e-001
16 1.059801531e-001
17 5.471024929e-002
18 -2.347296974e-003
19 -1.437903732e-001
20 1.207354980e-001

Total number of rows: 45015

Table truncated, full table size 1016 Kbytes.




Supplementary file Size Download File type/resource
GSM492576.txt.gz 14.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap