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Sample GSM492563 Query DataSets for GSM492563
Status Public on Apr 19, 2010
Title Integrative genomics of ovarian cancer P4616 [GenomeWideSNP_6]
Sample type genomic
 
Source name Ovarian tumours at the time of diagnosis
Organism Homo sapiens
Characteristics tissue type (t=tumour; n= normal blood lymphocyte): T&N
histological subtype: serous
grade: 3
stage: 1c
age: 56
scandate(batch): 4-16-08
source: Tissue_bank
Treatment protocol All tumours were fresh-frozen and OCT embedded. Lymphocytic DNA was extracted from stored blood samples.
Extracted molecule genomic DNA
Extraction protocol 10uM slices of tumour were hand-microdissected to obtain regions with >80% epithelial cells and reduce contamination from fibroblasts, blood vessels etc. Following this, for DNA extraction, slides containing sections were stained with Haematoxylin and Eosin and DNA was extracted using the Qiagen DNEasy kit. For RNA, identical slides were stained with Cresyl violet and total RNA was extracted using the Ambion mirVana kit according to manufacturer's instructions.
Label biotin
Label protocol According to Affymetrix protocol, Biotin labelled
 
Hybridization protocol According to Affymetrix protocol using recommended instrumentation
Scan protocol According to Affymetrix protocol and using GeneChip Operating Software
Description none
Data processing primary data description: Partek's copy number workflow generates copy number ratios relative to matching normal samples or a pooled baseline and then converts and presents these values as whole copy numbers. The file titled SNP6_Normalised-Copy-number.csv contains these values for each sample. In this file, probes on Chromosome Y and the Mitochondrion (MT) have been removed and the probesets are organised first by Chromosome and then by start position. Annotations for this were mapped in Partek using the Affymetrix GenomewideSNP6, CN and SNP annotation files, release number 23. This data was then used for Segmentation using the CBS algorithm in the DNAcopy package in R open-source software.
 
Submission date Dec 31, 2009
Last update date Jul 09, 2014
Contact name Manasa Ramakrishna
E-mail(s) manasa.ramakrishna@petermac.org
Organization name Peter MacCallum Cancer Centre
Street address St Andrew's Pl
City Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
 
Platform ID GPL6801
Series (1)
GSE19539 Identification of Novel Oncogene Loci in Ovarian Cancer through Integrated Copy Number and Expression Analysis
Relations
Affiliated with GSE58579

Data table header descriptions
ID_REF
VALUE ratio

Data table
ID_REF VALUE
CN_473963 1.2826999425888
CN_473964 1.2390600442886
CN_473965 1.3414800167084
CN_477984 1.8628499507904
CN_473981 1.6301300525665
CN_473982 1.0020799636841
CN_497980 1.2787799835205
CN_497981 1.5384299755096
CN_513369 0.28270000219345
CN_513370 1.7626299858093
CN_517762 1.3757699728012
CN_517763 1.9766399860382
CN_502615 0.72881197929382
CN_502613 0.71485501527786
CN_502614 0.23442600667477
CN_502616 0.28165999054909
CN_502842 1.0885499715805
CN_502843 1.5900100469589
CN_466171 1.4064699411392
CN_466172 2.4400000572205

Total number of rows: 1870003

Table truncated, full table size 51383 Kbytes.




Supplementary file Size Download File type/resource
GSM492563_P4616N.CEL.gz 29.5 Mb (ftp)(http) CEL
GSM492563_P4616T.CEL.gz 28.8 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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