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Sample GSM492513 Query DataSets for GSM492513
Status Public on Apr 19, 2010
Title Integrative genomics of ovarian cancer IC325 [GenomeWideSNP_6]
Sample type genomic
 
Source name Ovarian tumours at the time of diagnosis
Organism Homo sapiens
Characteristics tissue type (t=tumour; n= normal blood lymphocyte): T&N
histological subtype: serous
grade: 3
stage: -
age: 64
scandate(batch): 9-26-07
source: Campbell
Treatment protocol All tumours were fresh-frozen and OCT embedded. Lymphocytic DNA was extracted from stored blood samples.
Extracted molecule genomic DNA
Extraction protocol 10uM slices of tumour were hand-microdissected to obtain regions with >80% epithelial cells and reduce contamination from fibroblasts, blood vessels etc. Following this, for DNA extraction, slides containing sections were stained with Haematoxylin and Eosin and DNA was extracted using the Qiagen DNEasy kit. For RNA, identical slides were stained with Cresyl violet and total RNA was extracted using the Ambion mirVana kit according to manufacturer's instructions.
Label biotin
Label protocol According to Affymetrix protocol, Biotin labelled
 
Hybridization protocol According to Affymetrix protocol using recommended instrumentation
Scan protocol According to Affymetrix protocol and using GeneChip Operating Software
Description none
Data processing primary data description: Partek's copy number workflow generates copy number ratios relative to matching normal samples or a pooled baseline and then converts and presents these values as whole copy numbers. The file titled SNP6_Normalised-Copy-number.csv contains these values for each sample. In this file, probes on Chromosome Y and the Mitochondrion (MT) have been removed and the probesets are organised first by Chromosome and then by start position. Annotations for this were mapped in Partek using the Affymetrix GenomewideSNP6, CN and SNP annotation files, release number 23. This data was then used for Segmentation using the CBS algorithm in the DNAcopy package in R open-source software.
 
Submission date Dec 31, 2009
Last update date Jul 09, 2014
Contact name Manasa Ramakrishna
E-mail(s) manasa.ramakrishna@petermac.org
Organization name Peter MacCallum Cancer Centre
Street address St Andrew's Pl
City Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
 
Platform ID GPL6801
Series (1)
GSE19539 Identification of Novel Oncogene Loci in Ovarian Cancer through Integrated Copy Number and Expression Analysis
Relations
Affiliated with GSE58579

Data table header descriptions
ID_REF
VALUE ratio

Data table
ID_REF VALUE
CN_473963 1.9293899536133
CN_473964 2.6457800865173
CN_473965 2.2531499862671
CN_477984 1.7218600511551
CN_473981 2.2350299358368
CN_473982 2.4184799194336
CN_497980 2.4026200771332
CN_497981 2.1818299293518
CN_513369 4.4398498535156
CN_513370 1.6404000520706
CN_517762 2.2887699604034
CN_517763 2.9026999473572
CN_502615 1.8494700193405
CN_502613 3.287780046463
CN_502614 1.8129099607468
CN_502616 1.500550031662
CN_502842 2.2360799312592
CN_502843 1.2529100179672
CN_466171 1.2531199455261
CN_466172 2.6641700267792

Total number of rows: 1870003

Table truncated, full table size 51288 Kbytes.




Supplementary file Size Download File type/resource
GSM492513_IC325N.CEL.gz 33.5 Mb (ftp)(http) CEL
GSM492513_IC325T.CEL.gz 32.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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