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Sample GSM492508 Query DataSets for GSM492508
Status Public on Apr 19, 2010
Title Integrative genomics of ovarian cancer IC293 [GenomeWideSNP_6]
Sample type genomic
Source name Ovarian tumours at the time of diagnosis
Organism Homo sapiens
Characteristics tissue type (t=tumour; n= normal blood lymphocyte): T&N
histological subtype: endometrioid
grade: 3
stage: 1
age: 59
scandate(batch): 2-26-08
source: Campbell
Treatment protocol All tumours were fresh-frozen and OCT embedded. Lymphocytic DNA was extracted from stored blood samples.
Extracted molecule genomic DNA
Extraction protocol 10uM slices of tumour were hand-microdissected to obtain regions with >80% epithelial cells and reduce contamination from fibroblasts, blood vessels etc. Following this, for DNA extraction, slides containing sections were stained with Haematoxylin and Eosin and DNA was extracted using the Qiagen DNEasy kit. For RNA, identical slides were stained with Cresyl violet and total RNA was extracted using the Ambion mirVana kit according to manufacturer's instructions.
Label biotin
Label protocol According to Affymetrix protocol, Biotin labelled
Hybridization protocol According to Affymetrix protocol using recommended instrumentation
Scan protocol According to Affymetrix protocol and using GeneChip Operating Software
Description none
Data processing primary data description: Partek's copy number workflow generates copy number ratios relative to matching normal samples or a pooled baseline and then converts and presents these values as whole copy numbers. The file titled SNP6_Normalised-Copy-number.csv contains these values for each sample. In this file, probes on Chromosome Y and the Mitochondrion (MT) have been removed and the probesets are organised first by Chromosome and then by start position. Annotations for this were mapped in Partek using the Affymetrix GenomewideSNP6, CN and SNP annotation files, release number 23. This data was then used for Segmentation using the CBS algorithm in the DNAcopy package in R open-source software.
Submission date Dec 31, 2009
Last update date Apr 19, 2010
Contact name Manasa Ramakrishna
Organization name Peter MacCallum Cancer Centre
Street address St Andrew's Pl
City Melbourne
State/province Victoria
ZIP/Postal code 3002
Country Australia
Platform ID GPL6801
Series (1)
GSE19539 Identification of Novel Oncogene Loci in Ovarian Cancer through Integrated Copy Number and Expression Analysis

Data table header descriptions
VALUE ratio

Data table
CN_473963 3.5501599311829
CN_473964 1.6769499778748
CN_473965 3.3490700721741
CN_477984 4.4241199493408
CN_473981 1.5494099855423
CN_473982 7.1224999427795
CN_497980 1.5166599750519
CN_497981 1.4321399927139
CN_513369 1.7235399484634
CN_513370 2.7805800437927
CN_517762 2.509740114212
CN_517763 1.860170006752
CN_502615 1.0552300214767
CN_502613 2.4211900234222
CN_502614 3.803750038147
CN_502616 0.73235297203064
CN_502842 2.3988800048828
CN_502843 1.3092299699783
CN_466171 1.8658200502396
CN_466172 2.6137299537659

Total number of rows: 1870003

Table truncated, full table size 51399 Kbytes.

Supplementary file Size Download File type/resource
GSM492508_IC293N.CEL.gz 31.0 Mb (ftp)(http) CEL
GSM492508_IC293T.CEL.gz 31.4 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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