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Sample GSM4905097 Query DataSets for GSM4905097
Status Public on Sep 29, 2021
Title Reprogramming MEFs_10 dpi_-4OHT_rep3 [iPS3]
Sample type SRA
 
Source name Reprogramming MEFs_10 dpi_-4OHT
Organism Mus musculus
Characteristics strain background: mixed; C57BL/6, DBA/2, and ICR
genotype/variation: Oct4-EGFP transgenic mouse
Sex: male
developmental stage: E13.5 embryos
cell type: mouse embryonic fibroblasts (MEFs)
treatment: Mock administration
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cells using the AllPrep DNA/RNA Mini Kit (QIAGEN) according to the manufacturer’s instructions.
Standard polyA libraries were prepared using the KAPA Stranded mRNA-Seq Kit for Illumina (Kapa Biosystems).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
 
Description AKT-MER was not activated
iPS3_L001
Data processing Mapped to mouse transcriptome by Salmon
TPM were calculated using tximport
Supplementary_files_format_and_content: Comma separated value files include TPM values for each Samples
 
Submission date Nov 12, 2020
Last update date Sep 29, 2021
Contact name Yoichi Sekita
E-mail(s) ysekita@kitasato-u.ac.jp
Phone +81-42-778-9481
Organization name Kitasato University School of Science
Department Biosciences
Lab Stem Cell Biology
Street address 1-15-1 Kitasato, Minami-ku
City Sagamihara
State/province Kanagawa
ZIP/Postal code 252-0373
Country Japan
 
Platform ID GPL18480
Series (1)
GSE161344 AKT signaling promotes epigenetic reprogramming via upregulation of TET and its cofactor, alpha-ketoglutarate during iPSC generation.
Relations
BioSample SAMN16779439
SRA SRX9491577

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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