|
Status |
Public on Sep 29, 2021 |
Title |
Reprogramming MEFs_10 dpi_-4OHT_rep1 [iPS1] |
Sample type |
SRA |
|
|
Source name |
Reprogramming MEFs_10 dpi_-4OHT
|
Organism |
Mus musculus |
Characteristics |
strain background: mixed; C57BL/6, DBA/2, and ICR genotype/variation: Oct4-EGFP transgenic mouse Sex: male developmental stage: E13.5 embryos cell type: mouse embryonic fibroblasts (MEFs) treatment: Mock administration
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from cells using the AllPrep DNA/RNA Mini Kit (QIAGEN) according to the manufacturer’s instructions. Standard polyA libraries were prepared using the KAPA Stranded mRNA-Seq Kit for Illumina (Kapa Biosystems).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Description |
AKT-MER was not activated iPS1_L001
|
Data processing |
Mapped to mouse transcriptome by Salmon TPM were calculated using tximport Supplementary_files_format_and_content: Comma separated value files include TPM values for each Samples
|
|
|
Submission date |
Nov 12, 2020 |
Last update date |
Sep 29, 2021 |
Contact name |
Yoichi Sekita |
E-mail(s) |
ysekita@kitasato-u.ac.jp
|
Phone |
+81-42-778-9481
|
Organization name |
Kitasato University School of Science
|
Department |
Biosciences
|
Lab |
Stem Cell Biology
|
Street address |
1-15-1 Kitasato, Minami-ku
|
City |
Sagamihara |
State/province |
Kanagawa |
ZIP/Postal code |
252-0373 |
Country |
Japan |
|
|
Platform ID |
GPL18480 |
Series (1) |
GSE161344 |
AKT signaling promotes epigenetic reprogramming via upregulation of TET and its cofactor, alpha-ketoglutarate during iPSC generation. |
|
Relations |
BioSample |
SAMN16779441 |
SRA |
SRX9491575 |