|
| Status |
Public on Nov 10, 2020 |
| Title |
Fibroblast 18 month replicate 5 |
| Sample type |
SRA |
| |
|
| Source name |
PDGFR⍺ cardiac fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
tissue: PDGFR⍺ cardiac fibroblasts
cell type: PDGFRalpha cardiac fibroblasts
strain: C57Bl/6
age: 18-month old
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Animals post-natal day 1, 4 weeks, 14 weeks, 1 year and 1.5 years old were used for determination of gene expression analysis. PDGFR⍺ cardiac fibroblasts were isolated by flow cytometry from hearts of animals at the above respective ages to determine changes in gene expression in cardiac fibroblasts.
RNA was isolated from the heart tissue or cardiac fibroblasts using RNeasy Mini kit (Qiagen) and used to generate RNA-Seq libraries followed by sequencing using Illumina 4000 platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
F-18M-5
|
| Data processing |
The reads were mapped with STAR 2.5.3a to the mouse genome (mm10).
The counts for each gene were obtained by using –quantMode GeneCounts commands in STAR, and the other parameters during alignment were set to default.
Genome_build: mm10
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Nov 09, 2020 |
| Last update date |
Nov 10, 2020 |
| Contact name |
Anela Tosevska |
| E-mail(s) |
anelatosevska@gmail.com
|
| Organization name |
Medical Univrsity of Vienna
|
| Department |
Division of Rheumatology
|
| Street address |
Waehringer Guertel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE161078 |
Cardiac fibroblast proliferation rates and collagen expression mature early in life and do not change with advancing age [RNA-Seq] |
| GSE161079 |
Cardiac fibroblast proliferation rates and collagen expression mature early in life and do not change with advancing age |
|
| Relations |
| BioSample |
SAMN16708997 |
| SRA |
SRX9461375 |
