|
| Status |
Public on Oct 26, 2020 |
| Title |
s6_1ngFGF9 |
| Sample type |
SRA |
| |
|
| Source name |
Cultured germ cells p9-12
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Cultured germ cells
replicate: 1
treatment: 1ng/ml FGF9
passages: p9-12
|
| Treatment protocol |
Germ cell culture with mouse serum-free media + 20ng/ml GDNF + 150ng/ml GFRA1 + treatment
|
| Growth protocol |
Germ cell culture with mouse serum-free media + 20ng/ml GDNF + 150ng/ml GFRA1 + 1ng/ml FGF2 between passages 9 and 12.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using miRNAeasy Mini Kit (QIAGEN, Germany)
mRNA libraries were created using TruSeq Stranded mRNA kit (Illumina, USA)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
1ng/ml FGF9
|
| Data processing |
Salmon using GRCm38.primary_assembly.genome.fa
Genomic alignments using STAR
Differential gene expression lists were created via DESeq2
Genome_build: GRCm38
Supplementary_files_format_and_content: csv file containing contrasts between each treatment and the 0ng FGF control
|
| |
|
| Submission date |
Oct 25, 2020 |
| Last update date |
Jul 10, 2024 |
| Contact name |
Eoin Christopher Whelan |
| E-mail(s) |
ewhelan@vet.upenn.edu
|
| Phone |
2158988805
|
| Organization name |
University of Pennsylvania School of Veterinary Medicine
|
| Department |
Biomedical Sciences
|
| Lab |
Sasaki
|
| Street address |
3800 Spruce St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE160062 |
FGF9 Promotes Mouse Spermatogonial Stem Cell Proliferation Mediated by p38 MAPK Signaling [bulk RNA-seq] |
| GSE160063 |
FGF9 Promotes Mouse Spermatogonial Stem Cell Proliferation Mediated by p38 MAPK Signaling |
|
| Relations |
| BioSample |
SAMN16547533 |
| SRA |
SRX9358306 |
