|
| Status |
Public on Nov 18, 2020 |
| Title |
LCC9, biological rep3 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
LCC9 cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LCC9
tamoxifen resistance: tamoxifen-resistant
fulvestrant resistance: fulvestrant-resistant
|
| Treatment protocol |
MCF-7, LCC2 or LCC9 cells were resuspended with phenol red free IMEM supplemented with 5% charcoal-stripped FBS and 1% penicillin/streptomycin and seeded in T75 flasks. Culture medium was changed once on day 2. Cells were washed with normal saline twice and harvested on day 4.
|
| Growth protocol |
MCF-7, LCC2 and LCC9 cells were grown in phenol red free IMEM supplemented with 5% charcoal-stripped FBS and 1% penicillin/streptomycin in a cell culture incubator at 37 ℃ and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix miRNA-4 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
FUL-5
Gene expression data from LCC9 cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 23, 2020 |
| Last update date |
Nov 18, 2020 |
| Contact name |
Min Jiang |
| E-mail(s) |
jiangmin1023@suda.edu.cn
|
| Organization name |
The First Affiliated Hospital of Soochow University
|
| Street address |
No. 188, Shi Zi Road
|
| City |
Suzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
215006 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (2) |
| GSE159979 |
miRNA expression data from MCF-7, LCC2 and LCC9 cells |
| GSE159981 |
lncRNA, mRNA, miRNA and circRNA expression data from MCF-7, LCC2 and LCC9 cells |
|
