NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM4812353 Query DataSets for GSM4812353
Status Public on Oct 02, 2020
Title PHx 0hr_scRNA-seq
Sample type SRA
 
Source name liver hepatocytes
Organism Mus musculus
Characteristics strain: C57BL/6
time point: 0hr
protocol: PHx surgery
Treatment protocol 10-12 weeks old mice underwent partial hepatectomy surgery
Growth protocol Mice were fed with normal diet
Extracted molecule total RNA
Extraction protocol Hepatocytes were isolated using two-step collagenase perfusion method
Libraries were prepared according to the 10X Genomics Single Cell RNA version 2
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description healthy, undamaged liver
Data processing The Cell Ranger RNA software v3.0.0 was used for processing sequencing data
Cells with high mitochondrial contents (>50%) were excluded from downstream analysis
Genome_build: mm10
Supplementary_files_format_and_content: cellranger output contains files including a sparse matrix, gene feature annotation and cell barcodes
 
Submission date Oct 01, 2020
Last update date Oct 06, 2020
Contact name Anna Mae Diehl
Organization name Duke University
Street address Research Drive
City Durham
ZIP/Postal code 27710
Country USA
 
Platform ID GPL24247
Series (2)
GSE158866 Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scRNA-Seq]
GSE158874 Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration
Relations
BioSample SAMN16327574
SRA SRX9227827

Supplementary file Size Download File type/resource
GSM4812353_PHX_0_10X_barcodes.tsv.gz 206.6 Kb (ftp)(http) TSV
GSM4812353_PHX_0_10X_genes.tsv.gz 212.7 Kb (ftp)(http) TSV
GSM4812353_PHX_0_10X_matrix.mtx.gz 123.1 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap