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Sample GSM4804924 Query DataSets for GSM4804924
Status Public on Feb 23, 2023
Title ASTROCYTE #1 CO-CULTURED WITH LNCAP CELLS
Sample type RNA
 
Source name HUMAN_ASTROCYTE_ COCULTURED LNCAP CELLS
Organism Homo sapiens
Characteristics cell type: HUMAN ASTROCYTE
Extracted molecule total RNA
Extraction protocol RNEAsy mini kit Qiagen
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human gene 1.0 ST. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Genechip Scanner 3000 7G
Description GENE EXPRESSION FROM HUMAN ASTROCYTE CO-CULTURED WITH LNCAP CELLS
Data processing The data were analyzed with Expression console (Affymetrix) (RMA) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Sep 28, 2020
Last update date Feb 23, 2023
Contact name Pedro Nicolau Neto
E-mail(s) pedronicolau.n@gmail.com
Organization name INCA
Department PCM
Street address 37 Andre Cavalcanti Street
City Rio de Janeiro
State/province RJ
ZIP/Postal code 20231-050
Country Brazil
 
Platform ID GPL6244
Series (1)
GSE158643 EXPRESSION DATA OF PROSTATE CELL LINES DU145 AND LNCAP AND HUMAN ASTROCYTES CO-CULTURED

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
7892501 5.84515
7892502 4.82546
7892503 3.02641
7892504 8.71593
7892505 3.26495
7892506 4.54435
7892507 3.68606
7892508 3.72481
7892509 11.1267
7892510 3.2748
7892511 3.47165
7892512 6.90942
7892513 3.10601
7892514 8.83189
7892515 10.1492
7892516 4.14394
7892517 5.50816
7892518 2.84814
7892519 4.19488
7892520 9.26016

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM4804924_1_astro_lncap_HuGene-1_0-st-v1_.CEL.gz 4.0 Mb (ftp)(http) CEL
GSM4804924_1_astro_lncap_HuGene-1_0-st-v1_.lncap.rma-gene-default.chp.gz 256.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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