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Sample GSM4804923 Query DataSets for GSM4804923
Status Public on Feb 23, 2023
Title ASTROCYTE #1 CO-CULTURED WITH DU145 CELLS
Sample type RNA
 
Source name HUMAN_ASTROCYTE_ COCULTURED DU145 CELLS
Organism Homo sapiens
Characteristics cell type: HUMAN ASTROCYTE
Extracted molecule total RNA
Extraction protocol RNEAsy mini kit Qiagen
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human gene 1.0 ST. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Genechip Scanner 3000 7G
Description GENE EXPRESSION FROM HUMAN ASTROCYTE CO-CULTURED WITH DU145 CELLS
Data processing The data were analyzed with Expression console (Affymetrix) (RMA) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Sep 28, 2020
Last update date Feb 23, 2023
Contact name Pedro Nicolau Neto
E-mail(s) pedronicolau.n@gmail.com
Organization name INCA
Department PCM
Street address 37 Andre Cavalcanti Street
City Rio de Janeiro
State/province RJ
ZIP/Postal code 20231-050
Country Brazil
 
Platform ID GPL6244
Series (1)
GSE158643 EXPRESSION DATA OF PROSTATE CELL LINES DU145 AND LNCAP AND HUMAN ASTROCYTES CO-CULTURED

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
7892501 4.02598
7892502 4.69729
7892503 3.68177
7892504 8.4496
7892505 4.29116
7892506 4.40281
7892507 3.11856
7892508 5.3442
7892509 11.4093
7892510 2.73505
7892511 4.1548
7892512 7.92891
7892513 4.12825
7892514 5.3077
7892515 10.5345
7892516 4.39299
7892517 5.31709
7892518 2.6992
7892519 4.87913
7892520 8.83926

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM4804923_1_astro_du145_HuGene-1_0-st-v1_.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM4804923_1_astro_du145_HuGene-1_0-st-v1_.du145.rma-gene-default.chp.gz 256.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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