|
Status |
Public on Oct 21, 2021 |
Title |
CD8_Human_T-cell_Hashing_Phage |
Sample type |
SRA |
|
|
Source name |
Peripherial Blood Mononucler Cells
|
Organism |
Homo sapiens |
Characteristics |
donor #: 4 distinct cell type: CD8 T-cells ab treatment: CD8 with hash barcode
|
Treatment protocol |
NA
|
Growth protocol |
Cryopreserved cells were thawed in DMEM with 10% FBS and washed in media
|
Extracted molecule |
genomic DNA |
Extraction protocol |
NP40 lysis, see method sections for full details Custom 10x Genomics scATAC-seq
|
|
|
Library strategy |
OTHER |
Library source |
genomic |
Library selection |
other |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Fastqs were generated using CellRanger-ATAC mkfastq v1.1.0 Sequencing reads were aligned using CellRanger-ATAC count for the hg38 reference genome For Phage libraries, phage tag abundances were quantified per barcode using a custom unix script. Genome_build: hg38 Supplementary_files_format_and_content: Per-barcode count abundance for phage library including the ATAC singlecell QC metrics; accessible chromatin fragments per atac library
|
|
|
Submission date |
Sep 04, 2020 |
Last update date |
Oct 21, 2021 |
Contact name |
Caleb Lareau |
E-mail(s) |
lareauc@mskcc.org
|
Organization name |
Memorial Sloan Kettering
|
Street address |
417 E 68th St, Zuckerman - ZRC 1132
|
City |
New York |
State/province |
New York |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL18573 |
Series (2) |
GSE157478 |
Single cell analysis of protein epitopes and chromatin accessibility using PHAGE-ATAC [CD8_Hashing] |
GSE157486 |
Single cell analysis of protein epitopes and chromatin accessibility using PHAGE-ATAC |
|
Relations |
BioSample |
SAMN16058318 |
SRA |
SRX9075318 |