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Sample GSM4766851 Query DataSets for GSM4766851
Status Public on Oct 21, 2021
Title CD8_Human_T-cell_Hashing_Phage
Sample type SRA
Source name Peripherial Blood Mononucler Cells
Organism Homo sapiens
Characteristics donor #: 4 distinct
cell type: CD8 T-cells
ab treatment: CD8 with hash barcode
Treatment protocol NA
Growth protocol Cryopreserved cells were thawed in DMEM with 10% FBS and washed in media
Extracted molecule genomic DNA
Extraction protocol NP40 lysis, see method sections for full details
Custom 10x Genomics scATAC-seq
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina NextSeq 500
Data processing Fastqs were generated using CellRanger-ATAC mkfastq v1.1.0
Sequencing reads were aligned using CellRanger-ATAC count for the hg38 reference genome
For Phage libraries, phage tag abundances were quantified per barcode using a custom unix script.
Genome_build: hg38
Supplementary_files_format_and_content: Per-barcode count abundance for phage library including the ATAC singlecell QC metrics; accessible chromatin fragments per atac library
Submission date Sep 04, 2020
Last update date Oct 21, 2021
Contact name Caleb Lareau
Organization name Memorial Sloan Kettering
Street address 417 E 68th St, Zuckerman - ZRC 1132
City New York
State/province New York
ZIP/Postal code 10065
Country USA
Platform ID GPL18573
Series (2)
GSE157478 Single cell analysis of protein epitopes and chromatin accessibility using PHAGE-ATAC [CD8_Hashing]
GSE157486 Single cell analysis of protein epitopes and chromatin accessibility using PHAGE-ATAC
BioSample SAMN16058318
SRA SRX9075318

Supplementary file Size Download File type/resource
GSM4766851_CD8_Human_T-cell_Hashing_singlecell_counts.csv.gz 4.7 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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