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Sample GSM4725591 Query DataSets for GSM4725591
Status Public on Aug 13, 2020
Title WT_LPS2
Sample type SRA
Source name B cell
Organism Mus musculus
Characteristics Sex: female
strain: C57BL/6J
sorting markers: Thy1.2-F4/80-CD11b-Viability-CD45.1+CD45.2-
tissue: Spleen
stimulation: LPS
Treatment protocol B cells of the indicated genotype were harvested from the spleen 72 hours following LPS or NP-Ficoll innoculation.
Extracted molecule polyA RNA
Extraction protocol Adoptively transferred cells were FACS isolated and resuspended in 1xPBS at 1000 cells/ul for GEM loading.
GEM creation and library preparation was performed according to the 10x recommended protocol.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description WTLPS_UMI_matrix.tsv
Data processing Raw data was demultiplexed into cells and the data mapped to the mouse mm10 genome build using CellRanger v2.1.1.
Biological replicates for WT-LPS and WT-NPFicoll stimulated B cells were aggregated using the CellRanger aggr function with the default parameters.
Genome_build: mm10
Supplementary_files_format_and_content: UMI transcript counts for each cell (columns) and all detected genes (rows)
Submission date Aug 12, 2020
Last update date Aug 13, 2020
Contact name Chris Scharer
Organization name Emory University
Department Microbiology and Immunology
Lab Chris Scharer
Street address 1510 Clifton Rd, Suite 3086A
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platform ID GPL24247
Series (1)
GSE136376 scRNA-seq of T cell Independent B cell responses
BioSample SAMN15804613
SRA SRX8935670

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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