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Sample GSM4710433 Query DataSets for GSM4710433
Status Public on Oct 25, 2021
Title HUVECs senescent-2
Sample type SRA
Source name HUVECs
Organism Homo sapiens
Characteristics tissue: umbilical vein endothelial cells
condition: senescent
Treatment protocol We prepared two kinds of HUVECs; one under proliferation as "young", the other that underwent replicative senescence as "senescent",
Growth protocol Human umbilical vein endothelial cells(HUVECs) were cultured in Endothelial Cell Growth Medium-2(Lonza)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from young and senescent HUVECs using RNeasy Plus Micro Kit (Quiagen)
cDNA libraries were generated using a NEBNext Ultra RNA Library Prep Kit (New England BioLabs)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Description HUVECs senescent-2
Data processing libraries were analyzed on a Highseq1500(Illumina).
TopHat 2 (version 2.0.13) was used for mapping reads to the reference genome (Ensembl GRCh38/hg38) with annotation data downloaded from the Ensembl Asia website (URL
Expression of each transcript was quantified as the number of fragments per kilobase of transcript per million fragments mapped (FPKM), and expression was compared among 3 groups by Cuffdiff (included in Cufflinks version 2.2.1).
Genome_build: Ensembl GRCh38/hg38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
Submission date Aug 04, 2020
Last update date Oct 25, 2021
Contact name Goro Katsuumi
Organization name Juntendo University
Street address Hongo 2-1-1
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 111-8421
Country Japan
Platform ID GPL18460
Series (1)
GSE155680 RNA-seq analysis in young and senescent endothelial cells
BioSample SAMN15728526
SRA SRX8884500

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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