|
| Status |
Public on Jan 27, 2021 |
| Title |
MM.1S_DMSO_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
MM.1S_DMSO
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MM.1S
treatment: DMSO
|
| Treatment protocol |
RPMI-8226 and MM.1S cells treated with GSK126 (1 μM), UNC0638 (1 μM) or their combination (1 μM each) for 6 days.
|
| Growth protocol |
RPMI-8226 and MM.1S cells were cultured in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37ºC in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini Kits (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
MM.1S_DMSO_2
|
| Data processing |
Normalization and background subtraction for intra-array data were performed according to Agilent Feature Extraction Software (v 10.7) protocol. For inter-array normalization, 75% percentile shift was performed.
|
| |
|
| Submission date |
Jul 26, 2020 |
| Last update date |
Jan 27, 2021 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16699 |
| Series (1) |
| GSE155135 |
Dual inhibition of EZH2 and G9a suppresses multiple myeloma cell proliferation by regulating interferon signal and IRF4-MYC axis |
|
