|
| Status |
Public on Jan 05, 2024 |
| Title |
G1E-ER4 0h - rnaseq_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
G1E-ER4
|
| Organism |
Mus musculus |
| Characteristics |
cell type: G1E-ER4
treatment: 10 nM beta-estradiol for 0h
|
| Treatment protocol |
Differentiation was induced by adding beta-estradiol (Sigma, cat: E8875) at a final concentration of 10 nM for 24 hrs or as otherwise indicated
|
| Growth protocol |
G1ER4 cells were cultured in Isocove’s modified Dulbecco’s medium (IMDM) supplemented with 15% fetal calf serum (FCS), 100 units (U) /mL penicillin and 100 g/mL streptomycin (PS), 2 U/mL recombinant human erythropoietin (EPO), and 50 ng/ml mouse recombinant SCF or 1% conditioned medium from murine SCF producer cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
isolated total RNA, depleted ribosomal RNA using the Ribo-Zero rRNA Removal Kit (Illumina cat: MRZH11124), and added ERCC RNA Spike-In Mix 1 (ThermoFisher: cat: 4456740) based on cell number
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Alignment done using TopHat2 and Bowtie
mm9
rna abudnance
|
| |
|
| Submission date |
Jul 24, 2020 |
| Last update date |
Jan 05, 2024 |
| Contact name |
Jonathan Li |
| E-mail(s) |
iamjli@mit.edu
|
| Organization name |
Massachusetts Institute of Technology
|
| Lab |
Ernest Fraenkel
|
| Street address |
77 Massachusetts Ave
|
| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE155103 |
GATA Switch Enhancers Mark Dynamically Regulated Chromatin Interaction Nodes during Erythroid Maturation |
|
| Relations |
| BioSample |
SAMN15641172 |
| SRA |
SRX8820370 |
