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Sample GSM4671480 Query DataSets for GSM4671480
Status Public on Jan 01, 2021
Title SCAP at c, biological rep1
Sample type RNA
 
Source name dental (apical papilla)
Organism Homo sapiens
Characteristics treatment: control
Treatment protocol For osteogenic differentiation, when cells reached 80% confluence, the medium was changed to osteogenic medium containing 10 mM β-glycerolphosphate (Sigma-Aldrich, St. Louis, MO, USA), 10 nM dexamethasone (Sigma-Aldrich) and 50 μg/mL L-ascorbic acid (Sigma-Aldrich). The osteogenic medium was changed every 3 days.
Growth protocol Then cells were cultured in α-minimum essential medium (HyClone, Logan, UT, United States) supplemented with 20% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (HyClone) at 37°C in a 5% CO2 incubator. The medium was changed every 3 days.
Extracted molecule total RNA
Extraction protocol RNA was extracted by mirVanaTM RNA Isolation Kit (AM1561 ) following the manufacturer's instructions.
Label biotin
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 48C on GeneChip miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray Scanner 3000.
Description Gene expression data from SCAP without differentiation.
Data processing The data were analyzed with Expression Console ™ Software 1.3 using Affymetrix default analysis settings and global scaling as normalization method.The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jul 15, 2020
Last update date Jan 03, 2021
Contact name Junqing Liu
Organization name Shandong University
Street address No.44-1 Wenhua Road West
City Jinan
ZIP/Postal code 250012
Country China
 
Platform ID GPL19117
Series (1)
GSE154466 Expression data of microRNA from human stem cells from apical papilla

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
MIMAT0000001_st 3769.219
MIMAT0015091_st 1.208459
MIMAT0000002_st 1.330397
MIMAT0015092_st 2.539264
MIMAT0020301_st 1.16704
MIMAT0000003_st 1.311648
MIMAT0020302_st 1.30223
MIMAT0000004_st 1.037892
MIMAT0000005_st 4.240859
MIMAT0015093_st 1.264217
MIMAT0020303_st 0.916064
MIMAT0000006_st 1.921117
MIMAT0020304_st 1.19189
MIMAT0000007_st 1.197946
MIMAT0015094_st 1.291605
MIMAT0000008_st 1.026448
MIMAT0020305_st 1.180492
MIMAT0000009_st 1.445079
MIMAT0020306_st 1.062796
MIMAT0000010_st 1.10822

Total number of rows: 36249

Table truncated, full table size 878 Kbytes.




Supplementary file Size Download File type/resource
GSM4671480_160219-151168A_B_1c_miRNA-4_0_.CEL.gz 692.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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