|
Status |
Public on Nov 04, 2009 |
Title |
NFYA-siRNA transfected cells vs. mock transfected cells-siRNA1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
MDA-MB-231 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231 transfection: 72hr post-transfection with NFYA-siRNA
|
Growth protocol |
MDA-MB-231 cells were growin in D10F medium and were transfected using LipofectamineTM 2000 (invitrogen)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using mirVana™ miRNA Isolation Kit following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Samples labeled using Agilent Quick-Amp Labeling kit following manufacyurer's instructions
|
|
|
Channel 2 |
Source name |
MDA-MB-231 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231 transfection: 72hr after mock-transfection
|
Growth protocol |
MDA-MB-231 cells were growin in D10F medium and were transfected using LipofectamineTM 2000 (invitrogen)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using mirVana™ miRNA Isolation Kit following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
Samples labeled using Agilent Quick-Amp Labeling kit following manufacyurer's instructions
|
|
|
|
Hybridization protocol |
Samples were hybridized using Agilent's gene expression hybridization kit and wash buffers according to the manufacturer's protocol
|
Scan protocol |
Scanned on an Agilent G2565CA scanner. Images were quantified using Agilent Feature Extraction Software (version 9.5).
|
Description |
NFYA knock-down vs control
|
Data processing |
Agilent Feature Extraction Software (v 9.5) was used for data extraction.
|
|
|
Submission date |
Nov 02, 2009 |
Last update date |
Nov 03, 2009 |
Contact name |
Hani Goodarzi |
Organization name |
UCSF
|
Department |
Biochemistry and Biophysics
|
Street address |
600 16th St, GH S312D
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL4133 |
Series (2) |
GSE18849 |
ELK1 and NFYA knockdown in MBA-MD-231 cell line |
GSE18874 |
Decoy vs Scrambled and siRNA vs Mock Transfected MBA-MD-231 Cell Line |
|