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Sample GSM467136 Query DataSets for GSM467136
Status Public on Nov 04, 2009
Title NFYA-siRNA transfected cells vs. mock transfected cells-siRNA1
Sample type RNA
 
Channel 1
Source name MDA-MB-231 cell line
Organism Homo sapiens
Characteristics cell line: MDA-MB-231
transfection: 72hr post-transfection with NFYA-siRNA
Growth protocol MDA-MB-231 cells were growin in D10F medium and were transfected using LipofectamineTM 2000 (invitrogen)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using mirVana™ miRNA Isolation Kit following manufacturer's instructions
Label Cy3
Label protocol Samples labeled using Agilent Quick-Amp Labeling kit following manufacyurer's instructions
 
Channel 2
Source name MDA-MB-231 cell line
Organism Homo sapiens
Characteristics cell line: MDA-MB-231
transfection: 72hr after mock-transfection
Growth protocol MDA-MB-231 cells were growin in D10F medium and were transfected using LipofectamineTM 2000 (invitrogen)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using mirVana™ miRNA Isolation Kit following manufacturer's instructions
Label Cy5
Label protocol Samples labeled using Agilent Quick-Amp Labeling kit following manufacyurer's instructions
 
 
Hybridization protocol Samples were hybridized using Agilent's gene expression hybridization kit and wash buffers according to the manufacturer's protocol
Scan protocol Scanned on an Agilent G2565CA scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.5).
Description NFYA knock-down vs control
Data processing Agilent Feature Extraction Software (v 9.5) was used for data extraction.
 
Submission date Nov 02, 2009
Last update date Nov 03, 2009
Contact name Hani Goodarzi
Organization name UCSF
Department Biochemistry and Biophysics
Street address 600 16th St, GH S312D
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL4133
Series (2)
GSE18849 ELK1 and NFYA knockdown in MBA-MD-231 cell line
GSE18874 Decoy vs Scrambled and siRNA vs Mock Transfected MBA-MD-231 Cell Line

Data table header descriptions
ID_REF
VALUE Normalized log10 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
1 0.132265324
2 0
3 0
4 0
5 0
6 0
7 0
8 0
9 0
10 0
11 0
12 -0.011686583
13 0.019356848
14 0.029837457
15 -0.255846978
16 0.008366087
17 -0.183181619
18 -0.017790489
19 0.238690738
20 -0.137214922

Total number of rows: 45015

Table truncated, full table size 722 Kbytes.




Supplementary file Size Download File type/resource
GSM467136.txt.gz 15.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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