|
| Status |
Public on Aug 01, 2011 |
| Title |
n-21-1 |
| Sample type |
RNA |
| |
|
| Source name |
benign prostatic hyperplasia
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: benign prostatic hyperplasia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
purification from formalin-fixed paraffin embedded (FFPE) material (RecoverAll™ Total Nucleic Acid Isolation Kit), subsequent miRNA purification (flashPAGE™ Fractionator) without deviation from the original protocol
|
| Label |
Cy3
|
| Label protocol |
labeling according to the Applied Biosystems mirVana™ manual without deviation from the original protocol
|
| |
|
| Hybridization protocol |
hybridization for 16 hr at 42C using a Agilent hybridization oven, labeled targets from 15ug total RNA were used for hybridization
|
| Scan protocol |
Arrays were scanned using the ScanArray 4000 (PerkinElmer)
|
| Description |
mirRNA expression data from a benign prostatic hyperplasia isolated from patient n-21-1
|
| Data processing |
The data were analyzed with Bioconductor packages (under R) 1st median value of 4 replicates (flaged spots excluded) was calculated, 2nd vsn normalization, 3rd gene finding using the Limma-package
|
| |
|
| Submission date |
Oct 21, 2009 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Burkhard Gerhard Kneitz |
| E-mail(s) |
Kneitz_B@klinik.uni-wuerzburg.de
|
| Phone |
+49-931-201-32700
|
| Organization name |
University of Wuerzburg
|
| Department |
Klinik und Poliklinik für Urologie und Kinderurologie
|
| Street address |
Oberdürrbacher Str. 6
|
| City |
Wuerzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9173 |
| Series (1) |
| GSE18671 |
miRNA expression data from human high and low risk prostate carcinoma |
|
