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Sample GSM463755 Query DataSets for GSM463755
Status Public on Aug 01, 2011
Title l6-22-17
Sample type RNA
 
Source name prostate carcinoma
Organism Homo sapiens
Characteristics disease state: low risk prostate carcinoma
Extracted molecule total RNA
Extraction protocol purification from formalin-fixed paraffin embedded (FFPE) material (RecoverAll™ Total Nucleic Acid Isolation Kit), subsequent miRNA purification (flashPAGE™ Fractionator) without deviation from the original protocol
Label Cy3
Label protocol labeling according to the Applied Biosystems mirVana™ manual without deviation from the original protocol
 
Hybridization protocol hybridization for 16 hr at 42C using a Agilent hybridization oven, labeled targets from 15ug total RNA were used for hybridization
Scan protocol Arrays were scanned using the ScanArray 4000 (PerkinElmer)
Description Gene expression data from a low risk prostate carcinoma l6-22-17
Data processing The data were analyzed with Bioconductor packages (under R) 1st median value of 4 replicates (flaged spots excluded) was calculated, 2nd vsn normalization, 3rd gene finding using the Limma-package
 
Submission date Oct 21, 2009
Last update date Aug 01, 2011
Contact name Burkhard Gerhard Kneitz
E-mail(s) Kneitz_B@klinik.uni-wuerzburg.de
Phone +49-931-201-32700
Organization name University of Wuerzburg
Department Klinik und Poliklinik für Urologie und Kinderurologie
Street address Oberdürrbacher Str. 6
City Wuerzburg
ZIP/Postal code 97080
Country Germany
 
Platform ID GPL9173
Series (1)
GSE18671 miRNA expression data from human high and low risk prostate carcinoma

Data table header descriptions
ID_REF
VALUE calculation of the median value of 4 replicates (flaged spots excluded), followed by normalization of the Signal intensities with bioconductor package 'vsn'

Data table
ID_REF VALUE
1 8.0701
2 5.4865
3 5.3585
4 6.6237
9 5.6473
10 6.6276
11 6.9391
12 6.0011
17 5.7054
18 5.9694
19 5.5376
20 6.0708
25 7.1147
26 6.5674
27 7.6393
28 6.6554
65 6.1078
66 5.553
67 6.2424
68 6.3809

Total number of rows: 665

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM463755.DAT.gz 33.4 Kb (ftp)(http) DAT
Processed data included within Sample table

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