|
Status |
Public on Aug 01, 2011 |
Title |
l6-22-17 |
Sample type |
RNA |
|
|
Source name |
prostate carcinoma
|
Organism |
Homo sapiens |
Characteristics |
disease state: low risk prostate carcinoma
|
Extracted molecule |
total RNA |
Extraction protocol |
purification from formalin-fixed paraffin embedded (FFPE) material (RecoverAll™ Total Nucleic Acid Isolation Kit), subsequent miRNA purification (flashPAGE™ Fractionator) without deviation from the original protocol
|
Label |
Cy3
|
Label protocol |
labeling according to the Applied Biosystems mirVana™ manual without deviation from the original protocol
|
|
|
Hybridization protocol |
hybridization for 16 hr at 42C using a Agilent hybridization oven, labeled targets from 15ug total RNA were used for hybridization
|
Scan protocol |
Arrays were scanned using the ScanArray 4000 (PerkinElmer)
|
Description |
Gene expression data from a low risk prostate carcinoma l6-22-17
|
Data processing |
The data were analyzed with Bioconductor packages (under R) 1st median value of 4 replicates (flaged spots excluded) was calculated, 2nd vsn normalization, 3rd gene finding using the Limma-package
|
|
|
Submission date |
Oct 21, 2009 |
Last update date |
Aug 01, 2011 |
Contact name |
Burkhard Gerhard Kneitz |
E-mail(s) |
Kneitz_B@klinik.uni-wuerzburg.de
|
Phone |
+49-931-201-32700
|
Organization name |
University of Wuerzburg
|
Department |
Klinik und Poliklinik für Urologie und Kinderurologie
|
Street address |
Oberdürrbacher Str. 6
|
City |
Wuerzburg |
ZIP/Postal code |
97080 |
Country |
Germany |
|
|
Platform ID |
GPL9173 |
Series (1) |
GSE18671 |
miRNA expression data from human high and low risk prostate carcinoma |
|