NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM463751 Query DataSets for GSM463751
Status Public on Aug 01, 2011
Title h+22-7
Sample type RNA
 
Source name prostate carcinoma
Organism Homo sapiens
Characteristics disease state: high risk prostate carcinoma
Extracted molecule total RNA
Extraction protocol purification from formalin-fixed paraffin embedded (FFPE) material (RecoverAll™ Total Nucleic Acid Isolation Kit), subsequent miRNA purification (flashPAGE™ Fractionator) without deviation from the original protocol
Label Cy3
Label protocol labeling according to the Applied Biosystems mirVana™ manual without deviation from the original protocol
 
Hybridization protocol hybridization for 16 hr at 42C using a Agilent hybridization oven, labeled targets from 15ug total RNA were used for hybridization
Scan protocol Arrays were scanned using the ScanArray 4000 (PerkinElmer)
Description Gene expression data from a high risk prostate carcinoma h+22-7
Data processing The data were analyzed with Bioconductor packages (under R) 1st median value of 4 replicates (flaged spots excluded) was calculated, 2nd vsn normalization, 3rd gene finding using the Limma-package
 
Submission date Oct 21, 2009
Last update date Aug 01, 2011
Contact name Burkhard Gerhard Kneitz
E-mail(s) Kneitz_B@klinik.uni-wuerzburg.de
Phone +49-931-201-32700
Organization name University of Wuerzburg
Department Klinik und Poliklinik für Urologie und Kinderurologie
Street address Oberdürrbacher Str. 6
City Wuerzburg
ZIP/Postal code 97080
Country Germany
 
Platform ID GPL9173
Series (1)
GSE18671 miRNA expression data from human high and low risk prostate carcinoma

Data table header descriptions
ID_REF
VALUE calculation of the median value of 4 replicates (flaged spots excluded), followed by normalization of the Signal intensities with bioconductor package 'vsn'

Data table
ID_REF VALUE
1 7.4548
2 5.5551
3 5.2667
4 6.6027
9 5.3747
10 6.3669
11 7.404
12 5.9391
17 5.6513
18 5.6266
19 5.11
20 5.909
25 6.5952
26 6.001
27 8.346
28 6.7472
65 6.193
66 5.4003
67 5.9923
68 6.2092

Total number of rows: 665

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM463751.DAT.gz 32.8 Kb (ftp)(http) DAT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap