|
Status |
Public on May 17, 2010 |
Title |
CHM099 |
Sample type |
genomic |
|
|
Source name |
complete hydatidiform mole
|
Organism |
Homo sapiens |
Characteristics |
tissue: complete hydatidiform mole
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from complete hydatidiform mole tissues using the Qiagen DNA Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
|
Label |
biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
Scan protocol |
The arrays were washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
Description |
none
|
Data processing |
CEL files were processed by Affymetrix Genotyping Console 3.0.1. Calls were generated using birdseed-v2 algorithm. Copy number analysis was performed using "Reference Model File Creation and Analysis" mode without with regional GC correction configuration.
|
|
|
Submission date |
Oct 20, 2009 |
Last update date |
May 17, 2010 |
Contact name |
Kenshi Hayashi |
E-mail(s) |
hayashi.kenshi@gmail.com
|
Phone |
+81-92-642-6171
|
Organization name |
Kyushu University
|
Department |
Medical Institute of Bioregulation
|
Street address |
Maidashi 3-1-1, Higashi-ku
|
City |
Fukuoka |
ZIP/Postal code |
812-8582 |
Country |
Japan |
|
|
Platform ID |
GPL6801 |
Series (2) |
GSE18642 |
Definitive SNP/CNV haplotype map of Asians determined using a collection of complete hydatidiform moles (Affymetrix) |
GSE18701 |
Definitive SNP/CNV haplotype map of Asians determined using a collection of complete hydatidiform moles |
|