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Sample GSM4590017 Query DataSets for GSM4590017
Status Public on Jun 04, 2020
Title D1S2.120816
Sample type SRA
Source name Human Postmortem Brain
Organism Homo sapiens
Characteristics platform: Dropseq
tissue: Neocortex; V1
Extracted molecule total RNA
Extraction protocol
Drop-seq (Macosko et al. 2014 Cell) 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Description single-nucleus gene expression data based on 3' transcript counts
Data processing Sequencing Reads were aligned against the relevant genome build using STAR
Aligned Reads within a gene were collapsed to UMI(XM)-Gene Pairs and associated with single-cells through a cell barcode (XC) shared on Read 1
A count matrix - called the digital gene expression (DGE) matrix - was output for GAD1+/GAD2+ interneurons. Columns belong to STAMPs (cells) and transcript counts (rows)
Genome_build: GRCm38.81 (mouse); MusPutFur1.0 (ferret); calJac3 (marmoset); rheMac8 (macaque); hg19 (human)
Supplementary_files_format_and_content: digital gene expression matrix; comma-delimited file with columns = cells and rows = transcript counts
Submission date Jun 03, 2020
Last update date Apr 20, 2022
Contact name Fenna Marie Krienen
Organization name Harvard Medical School
Department Genetics
Lab McCarroll
Street address 77 Ave Louis Pasteur, NRB 260
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18573
Series (1)
GSE151761 Innovations in primate interneuron repertoire
BioSample SAMN15096864

Supplementary data files not provided
Processed data are available on Series record
Raw data not provided for this record

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