|
| Status |
Public on May 29, 2023 |
| Title |
Patient 1 HGF |
| Sample type |
RNA |
| |
|
| Source name |
Scalp skin
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
agent: HGF
|
| Treatment protocol |
The hair follicles were grown with/without recombinant human HGF protein.
|
| Growth protocol |
Human scalp hair follicles were cultured ex vivo for 6 hours in William's E Media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNeasy Micro Kit.
|
| Label |
Cy3
|
| Label protocol |
Samples were prepared as per the Agilent One-Colour Microarray-Based Gene Expression Analysis Protocol (version 6.9.1) using the Amp Labelling Kit
|
| |
|
| Hybridization protocol |
Samples were hybridized as per the Agilent One-Colour Microarray-Based Gene Expression Analysis Protocol (version 6.9.1) using Agilent hi-RPM Gene Expression hybridization kit reagents.
|
| Scan protocol |
Slides were scanned using the Agilent Technologies microarray scanner G2505C US84700251.
|
| Description |
Donor 1 HGF
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters (protocol GE1_1200_Jun14) to obtain background subtracted and spatially de-trended Processed Signal intensities – the final data is presented as gProcessed signal. Filtering and analysis of data was carried out using GeneSpring GX13: data was per sample normalized by percentile shift to the 75th percentile, and baseline transformed to the time matched vehicle control sample from the same donor.
|
| |
|
| Submission date |
Jun 01, 2020 |
| Last update date |
May 29, 2023 |
| Contact name |
Carina Nicu |
| E-mail(s) |
cxn467@miami.edu
|
| Phone |
001-305-243-4721
|
| Organization name |
University of Miami
|
| Department |
Dr Phillip Frost Dept of Dermatology and Cutaneous Surgery
|
| Lab |
Prof. Ralf Paus's Lab
|
| Street address |
1600 NW 10th Ave RMSB 6157 Locator Code R-250
|
| City |
Miami |
| State/province |
Florida |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL21185 |
| Series (1) |
| GSE151560 |
Profiling the response of scalp hair follicles to short-term treatment with hepatocyte growth factor |
|
