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Sample GSM4566514 Query DataSets for GSM4566514
Status Public on Jun 15, 2021
Title Sample 00405 (Lung, Tumor)
Sample type RNA
 
Source name Lung
Organism Homo sapiens
Characteristics diagnosis: Lung cancer
gender: Female
tissue: Tumor
patient id: 00405
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
Label Biotin
Label protocol Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
 
Hybridization protocol Hybridization was performed for 16 h on GeneTitanTM Instrument.
Scan protocol GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
Description G7_00405_T_RL
Gene expression from surgically resected lung tissue in lung cancer patients.
Data processing Raw expression data for these 237 samples were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
 
Submission date May 23, 2020
Last update date Nov 16, 2021
Contact name William Cookson
E-mail(s) w.cookson@imperial.ac.uk
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
 
Platform ID GPL11532
Series (2)
GSE151101 Disruption of Y chromosome expression in male lung cancer: discovery
GSE151103 Disruption of Y chromosome expression in male lung cancer

Data table header descriptions
ID_REF
VALUE RMA normalised transcript cluster level expression values

Data table
ID_REF VALUE
7896759 4.23032364
7896761 4.038570528
7896779 5.025264465
7896798 5.072896663
7896817 5.876825882
7896822 6.917263348
7896859 3.016055687
7896865 4.692811899
7896878 5.421502182
7896882 4.390881576
7896908 6.115010474
7896952 6.399362832
7896985 5.355749566
7897034 4.704004434
7897044 6.323341481
7897066 4.156145795
7897068 8.578423375
7897078 7.171256353
7897089 4.732323131
7897119 5.55909954

Total number of rows: 18717

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM4566514_G7_00405_T_RL.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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