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Sample GSM4566507 Query DataSets for GSM4566507
Status Public on Jun 15, 2021
Title Sample 52 (Lung, Tumor)
Sample type RNA
 
Source name Lung
Organism Homo sapiens
Characteristics diagnosis: Lung cancer
gender: Male
tissue: Tumor
patient id: 52
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
Label Biotin
Label protocol Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
 
Hybridization protocol Hybridization was performed for 16 h on GeneTitanTM Instrument.
Scan protocol GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
Description G5_52-T-RL
Gene expression from surgically resected lung tissue in lung cancer patients.
Data processing Raw expression data for these 237 samples were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
 
Submission date May 23, 2020
Last update date Nov 16, 2021
Contact name William Cookson
E-mail(s) w.cookson@imperial.ac.uk
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
 
Platform ID GPL11532
Series (2)
GSE151101 Disruption of Y chromosome expression in male lung cancer: discovery
GSE151103 Disruption of Y chromosome expression in male lung cancer

Data table header descriptions
ID_REF
VALUE RMA normalised transcript cluster level expression values

Data table
ID_REF VALUE
7896759 3.792013787
7896761 4.134879624
7896779 4.856105738
7896798 4.852709526
7896817 4.320663135
7896822 7.548614646
7896859 3.328469853
7896865 4.585267347
7896878 5.421804356
7896882 4.009430525
7896908 5.965208347
7896952 6.390000084
7896985 5.51200191
7897034 4.62606163
7897044 7.844510911
7897066 4.114029974
7897068 8.077489934
7897078 7.686516645
7897089 5.947271409
7897119 5.437134131

Total number of rows: 18717

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM4566507_G5_52-T-RL.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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