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Sample GSM4566455 Query DataSets for GSM4566455
Status Public on Jun 15, 2021
Title Sample 00271 (Lung, Tumor)
Sample type RNA
 
Source name Lung
Organism Homo sapiens
Characteristics diagnosis: Lung cancer
gender: Male
tissue: Tumor
patient id: 00271
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
Label Biotin
Label protocol Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
 
Hybridization protocol Hybridization was performed for 16 h on GeneTitanTM Instrument.
Scan protocol GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
Description E7_00271_T_RL
Gene expression from surgically resected lung tissue in lung cancer patients.
Data processing Raw expression data for these 237 samples were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
 
Submission date May 23, 2020
Last update date Nov 16, 2021
Contact name William Cookson
E-mail(s) w.cookson@imperial.ac.uk
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
 
Platform ID GPL11532
Series (2)
GSE151101 Disruption of Y chromosome expression in male lung cancer: discovery
GSE151103 Disruption of Y chromosome expression in male lung cancer

Data table header descriptions
ID_REF
VALUE RMA normalised transcript cluster level expression values

Data table
ID_REF VALUE
7896759 5.635507819
7896761 3.929263032
7896779 4.354200582
7896798 4.322487138
7896817 4.069710669
7896822 6.909994125
7896859 2.985443393
7896865 4.33238406
7896878 4.532737202
7896882 4.572266123
7896908 5.546060592
7896952 5.730642677
7896985 4.580954257
7897034 3.629773768
7897044 7.180011742
7897066 3.922234654
7897068 8.20718754
7897078 7.550897815
7897089 4.650513705
7897119 4.144405051

Total number of rows: 18717

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM4566455_E7_00271_T_RL.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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