|
Status |
Public on Jun 15, 2021 |
Title |
Sample 631 (Lung, Normal) |
Sample type |
RNA |
|
|
Source name |
Lung
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: Lung cancer gender: Male tissue: Normal patient id: 631
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
|
Label |
Biotin
|
Label protocol |
Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
|
|
|
Hybridization protocol |
Hybridization was performed for 16 h on GeneTitanTM Instrument.
|
Scan protocol |
GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
|
Description |
B9_631_N_RL Gene expression from surgically resected lung tissue in lung cancer patients.
|
Data processing |
Raw expression data for these 237 samples were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
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|
|
Submission date |
May 23, 2020 |
Last update date |
Nov 16, 2021 |
Contact name |
William Cookson |
E-mail(s) |
w.cookson@imperial.ac.uk
|
Organization name |
Imperial College London
|
Street address |
Dovehouse Street
|
City |
London |
ZIP/Postal code |
SW3 6LY |
Country |
United Kingdom |
|
|
Platform ID |
GPL11532 |
Series (2) |
GSE151101 |
Disruption of Y chromosome expression in male lung cancer: discovery |
GSE151103 |
Disruption of Y chromosome expression in male lung cancer |
|