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Sample GSM4566374 Query DataSets for GSM4566374
Status Public on Jun 15, 2021
Title Sample 606 (Lung, Normal)
Sample type RNA
 
Source name Lung
Organism Homo sapiens
Characteristics diagnosis: Lung cancer
gender: Male
tissue: Normal
patient id: 606
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
Label Biotin
Label protocol Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
 
Hybridization protocol Hybridization was performed for 16 h on GeneTitanTM Instrument.
Scan protocol GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
Description B7_606_N_RL
Gene expression from surgically resected lung tissue in lung cancer patients.
Data processing Raw expression data for these 237 samples were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
 
Submission date May 23, 2020
Last update date Nov 16, 2021
Contact name William Cookson
E-mail(s) w.cookson@imperial.ac.uk
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
 
Platform ID GPL11532
Series (2)
GSE151101 Disruption of Y chromosome expression in male lung cancer: discovery
GSE151103 Disruption of Y chromosome expression in male lung cancer

Data table header descriptions
ID_REF
VALUE RMA normalised transcript cluster level expression values

Data table
ID_REF VALUE
7896759 5.029178624
7896761 4.276385192
7896779 4.260113769
7896798 4.655336638
7896817 4.516813138
7896822 6.957125348
7896859 2.983141246
7896865 4.560498864
7896878 5.126717664
7896882 4.347353932
7896908 5.93247432
7896952 5.781967296
7896985 5.11016344
7897034 4.151023532
7897044 7.301845582
7897066 4.325731854
7897068 9.036272903
7897078 7.546938419
7897089 4.970849991
7897119 5.116626396

Total number of rows: 18717

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM4566374_B7_606_N_RL.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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