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Sample GSM4566366 Query DataSets for GSM4566366
Status Public on Jun 15, 2021
Title Sample 885 (Lung, Normal)
Sample type RNA
 
Source name Lung
Organism Homo sapiens
Characteristics diagnosis: Lung cancer
gender: Female
tissue: Normal
patient id: 885
Extracted molecule total RNA
Extraction protocol RNA was extracted using the Qiagen RNeasy mini kit (Qiagen, Crawley, UK).
Label Biotin
Label protocol Total RNA was converted to labelled cDNA for hybridization onto the gene expression chips using the AmbionTM WT Expression Kit. The single-stranded DNA was fragmented and labelled using Affymetrix GeneChipTM WT Terminal Labelling Kit.
 
Hybridization protocol Hybridization was performed for 16 h on GeneTitanTM Instrument.
Scan protocol GeneChips were scanned on a GeneTitan system (Affymetrix Ltd.).
Description B5_885_N_RL
Gene expression from surgically resected lung tissue in lung cancer patients.
Data processing Raw expression data for these 237 samples were RMA-treated using Oligo (1.38.0) as part of a larger study and filtered. Specifically, transcript cluster intensity was required to exceed the data set median in 1 or more sample (genefilter 1.56.0), and be designated within the Affymetrix annotation (netaffx build 36) with a cross-hybridisation potential of 1 (unique), a non-missing mRNA assignment and as part of the main design probe set category.
 
Submission date May 23, 2020
Last update date Nov 16, 2021
Contact name William Cookson
E-mail(s) w.cookson@imperial.ac.uk
Organization name Imperial College London
Street address Dovehouse Street
City London
ZIP/Postal code SW3 6LY
Country United Kingdom
 
Platform ID GPL11532
Series (2)
GSE151101 Disruption of Y chromosome expression in male lung cancer: discovery
GSE151103 Disruption of Y chromosome expression in male lung cancer

Data table header descriptions
ID_REF
VALUE RMA normalised transcript cluster level expression values

Data table
ID_REF VALUE
7896759 4.69597463
7896761 4.433428863
7896779 4.323498993
7896798 4.415336221
7896817 3.974827065
7896822 6.92845253
7896859 3.495683621
7896865 5.52697919
7896878 5.279858099
7896882 4.413068287
7896908 5.719993526
7896952 5.559749915
7896985 5.354339096
7897034 4.018514666
7897044 7.349129617
7897066 4.442446138
7897068 9.052582718
7897078 7.348845625
7897089 5.370360181
7897119 5.434117634

Total number of rows: 18717

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM4566366_B5_885_N_RL.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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