|
| Status |
Public on Mar 18, 2021 |
| Title |
Nelfb-KO_3 |
| Sample type |
SRA |
| |
|
| Source name |
Muscle satellite cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue type: Skeletal Muscle
cell type: Myogenic progenitor (TdT+, ITGA7+)
genotype: Nelfb-KO
|
| Treatment protocol |
Mice were induced with tamoxifen, then skeletal muscle damage through acute intramuscular cardiotoxin injection.
|
| Growth protocol |
Myoblasts were harvested from muscle 72h post injury and immediately purified by FACS (TdTomato+, Alpha-integrin 7+). Myoblasts were kept on ice when possible.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Myoblasts were prepared for single-cell RNA-sequencing using a 10 X genomics Chromium Single Cell 3' Reagent kit (cat. No. pn-1000075), according to the manufacturer protocol
Sequencing library were constructed using the 10 X genomics Chromium Single cell 3'v2.2 and 3'v3 Reagent kit (cat. No. pn-1000075), according to the manufacturer protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
cellranger mkfastq v2.1.1; performed on 10X Cell ranger pipeline
cellranger count v2.1.1
Genome_build: mm10
Supplementary_files_format_and_content: .mtx, .tsv: Processed contains single-cell gene expression matrix
|
| |
|
| Submission date |
May 11, 2020 |
| Last update date |
Dec 02, 2023 |
| Contact name |
Jeffrey Dilworth |
| E-mail(s) |
fdilworth@wisc.edu
|
| Phone |
6082653758
|
| Organization name |
University of Wisconsin
|
| Street address |
1111 Highland Ave
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE150278 |
Negative Elongation Factor (NELF) regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation [single-cell RNA] |
| GSE150280 |
Negative Elongation Factor (NELF) regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation |
|
| Relations |
| BioSample |
SAMN14888052 |
| SRA |
SRX8321672 |
