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Sample GSM4544638 Query DataSets for GSM4544638
Status Public on Mar 18, 2021
Title Nelfb-KO_3
Sample type SRA
 
Source name Muscle satellite cells
Organism Mus musculus
Characteristics strain: C57BL/6
tissue type: Skeletal Muscle
cell type: Myogenic progenitor (TdT+, ITGA7+)
genotype: Nelfb-KO
Treatment protocol Mice were induced with tamoxifen, then skeletal muscle damage through acute intramuscular cardiotoxin injection.
Growth protocol Myoblasts were harvested from muscle 72h post injury and immediately purified by FACS (TdTomato+, Alpha-integrin 7+). Myoblasts were kept on ice when possible.
Extracted molecule total RNA
Extraction protocol Myoblasts were prepared for single-cell RNA-sequencing using a 10 X genomics Chromium Single Cell 3' Reagent kit (cat. No. pn-1000075), according to the manufacturer protocol
Sequencing library were constructed using the 10 X genomics Chromium Single cell 3'v2.2 and 3'v3 Reagent kit (cat. No. pn-1000075), according to the manufacturer protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing cellranger mkfastq v2.1.1; performed on 10X Cell ranger pipeline
cellranger count v2.1.1
Genome_build: mm10
Supplementary_files_format_and_content: .mtx, .tsv: Processed contains single-cell gene expression matrix
 
Submission date May 11, 2020
Last update date Dec 02, 2023
Contact name Jeffrey Dilworth
E-mail(s) fdilworth@wisc.edu
Phone 6082653758
Organization name University of Wisconsin
Street address 1111 Highland Ave
City Madison
State/province Wisconsin
ZIP/Postal code 53705
Country USA
 
Platform ID GPL19057
Series (2)
GSE150278 Negative Elongation Factor (NELF) regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation [single-cell RNA]
GSE150280 Negative Elongation Factor (NELF) regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation
Relations
BioSample SAMN14888052
SRA SRX8321672

Supplementary file Size Download File type/resource
GSM4544638_Dilworth_Sample_KO-3.tar.gz 38.1 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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