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Sample GSM454293 Query DataSets for GSM454293
Status Public on Jan 01, 2010
Title Pancreas of E15.5 WT_rep3
Sample type RNA
 
Source name WT Pancreas, E15.5 embryo
Organism Mus musculus
Characteristics strain: C57BL/6
age: E15.5
tissue: pancreas
Extracted molecule total RNA
Extraction protocol PicoPure RNA Isolation kit with DNAseI treatment
Label biotin
Label protocol Starting with 100ng of total RNA, biotin-labeled cRNA was produced using the Affymetrix 3í Amplification Two-Cycle Target labeling kit according to manufacturerís protocol.
 
Hybridization protocol 15ug of amplified cRNAs were fragmented and hybridized to the array for 16 hours in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol.
Scan protocol Slides were stained and washed as indicated in the Antibody Amplification Stain for Eukaryotic Targets protocol using the Affymetrix Fluidics Station FS450. Arrays were then scanned with an Affymetrix Scanner 3000 and data was obtained using the GenechipÆ Operating Software (Version 1.4.0.036).
Description RNA sample was purified from WT embryo stage at 15.5 day
Data processing The resulting files (.dat, .cel and .chp) were imported into the Rosetta Resolver system (Version 7.2). This system performs data pre-processing and error modeling as described in Weng (2006). Resolver generated fold-changes and p values, based on ratios built in the system, were exported for further analysis.
 
Submission date Sep 18, 2009
Last update date Apr 06, 2011
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL1261
Series (1)
GSE18172 T.F. Glis3: a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression

Data table header descriptions
ID_REF probeset IDs from the Affymetrix Mouse430_2 genome array
VALUE normalized signal

Data table
ID_REF VALUE
1457007_at 101.47864
1427071_at 215.42169
1449287_at 99.98401
1419540_at
1418105_at
1425864_a_at
1426408_at 996.9668
1436645_a_at 1558.30725
1425040_at 175.55164
1416226_at 1557.01147
1446287_at
1439371_x_at 2912.32056
1435933_at
1438663_at 878.00922
1416397_at 235.70221
1419623_at
1437117_at 213.72617
1433792_at 471.9003
1450037_at 132.24152
1432628_at

Total number of rows: 45037

Table truncated, full table size 761 Kbytes.




Supplementary file Size Download File type/resource
GSM454293_8173_461_WT_Rep3.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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