NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM454279 Query DataSets for GSM454279
Status Public on Sep 23, 2009
Title WT3_mock
Sample type RNA
 
Source name Mouse/ Lung, WT, viral-free media treated
Organism Mus musculus
Characteristics gender: Male
strain: WT B6.129PF1/J
Extracted molecule total RNA
Extraction protocol Trizol/Qiagen RNA clean up
Label Cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
 
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description 24 hr infection
Data processing Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
 
Submission date Sep 18, 2009
Last update date Sep 22, 2009
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL7202
Series (1)
GSE18170 Double-Stranded RNA-Activated Protein Kinase Regulates early innate immune responses during RSV Infection

Data table header descriptions
ID_REF Agilent probe IDs from the Mouse Genome 4x44 array
VALUE Intensities of samples exported from Rosetta Resolver

Data table
ID_REF VALUE
A_52_P305654 222.92349
A_52_P891296 29.19564
A_51_P243283 62058.10156
A_52_P62270 249.17081
A_51_P362627 1146.89197
A_52_P229709 3501.28906
A_52_P662142 7.41946
A_52_P112017 27829.05078
A_51_P163444 8042.64697
A_51_P270605 6.38438
A_51_P239367 7.00746
A_51_P442759 212.16969
A_51_P106443 77.53237
A_51_P460247 48.56044
A_51_P275658 229.8076
A_52_P1044206 6.42073
A_52_P473542 2480.63208
A_51_P145507 67.83866
A_52_P219216 15.31565
A_52_P441355 6.52185

Total number of rows: 41174

Table truncated, full table size 903 Kbytes.




Supplementary file Size Download File type/resource
GSM454279_US22502532_251486814790_S01_GE1-v5_95_Feb07_1_3.txt.gz 9.1 Mb (ftp)(http) TXT
GSM454279_US22502532_251486814790_S01_H.tif.gz 62.5 Mb (ftp)(http) TIFF
GSM454279_US22502532_251486814790_S01_L.tif.gz 48.5 Mb (ftp)(http) TIFF
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap