GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM4505103 Query DataSets for GSM4505103
Status Public on Feb 24, 2021
Title SAL122_1876264_WT_Tumor Treg_rep4
Sample type RNA
Source name Treg cells from the B16 melanoma-bearing mice
Organism Mus musculus
Characteristics background strain: C57BL/6
cell type: Foxp3-YFP+ cells
cell population: tumor infiltrating Treg
tissue source: B16 melanoma tumor
genotype: WT
Treatment protocol Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
Growth protocol Tregs were sorted from lymph node/tumor from the B16 melanoma tumor and MC38 colon adenocarcinoma, and Tregs were sorted from pheripheral lymph node from WT mice.
Extracted molecule total RNA
Extraction protocol Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
Label biotin
Label protocol Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
Hybridization protocol Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Clariom S mouse GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
Description sgRNA transduced OT-I cells
Data processing Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
Submission date Apr 29, 2020
Last update date Feb 26, 2021
Contact name Hongbo Chi
Organization name St Jude Children's Research Hospital
Department Immunology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platform ID GPL23038
Series (2)
GSE149573 Lipid signalling enforces functional specialization of Treg cells in tumours [microarray #1]
GSE165259 Lipid signalling enforces functional specialization of Treg cells in tumours

Data table header descriptions
VALUE log2 RMA signal

Data table
ID_REF VALUE 8.776808 6.251683 8.84502 5.463118 4.943837 9.998515 8.747005 9.254858 7.630046 7.567931 6.30406 8.367456 7.732968 5.472394 6.228821 5.856688 5.497849 10.74233 9.506368 8.386008

Total number of rows: 28846

Table truncated, full table size 777 Kbytes.

Supplementary file Size Download File type/resource
GSM4505103_SAL122_1876264_ClariomSmouse.CEL.gz 1.2 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap