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Sample GSM449768 Query DataSets for GSM449768
Status Public on Sep 11, 2009
Title HCV+cirrhosis without HCC 28
Sample type RNA
 
Source name liver tissue
Organism Homo sapiens
Characteristics disease state: HCV + cirrhosis without HCC
tissue: liver
Treatment protocol Five mm frozen section slides were made and stained with H&E for Pathologist evaluation to determine tumor content. After Pathologist evaluation, normal/necrotic tissue was macrodissected. Twenty 20 mm sections were performed in a 15 ml tube with 10 ml of Trizol.
Growth protocol Fresh tissue specimens were collected from the consented patients in the operating room and processed immediately after extraction to minimize RNA degradation. Liver tissue samples were collected in liquid nitrogen or RNA later solution (Ambion, Inc, Austin, TX), and stored at -80┬░C until use.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.The precipitated product was further purified using RNeasy Mini Kit (Qiagen, CA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip® Scanner 3000 by Affymetrix.
Description Gene expression using RMA method
Data processing The RMA method in the R affy package was used to obtain probe set expression summaries.
 
Submission date Sep 04, 2009
Last update date Sep 10, 2009
Contact name Kellie J. Archer
E-mail(s) archer.43@osu.edu
Phone (614) 247-6167
Organization name Ohio State University
Department Division of Biostatistics
Street address 1841 Neil Ave
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
 
Platform ID GPL571
Series (1)
GSE17967 RMA expression data for liver samples from subjects with HCV cirrhosis with and without concomitant HCC

Data table header descriptions
ID_REF
VALUE Robust multiarray average (RMA)

Data table
ID_REF VALUE
1007_s_at 7.259930162
1053_at 5.804889637
117_at 5.125220022
121_at 6.994424052
1255_g_at 4.100605951
1294_at 6.840384679
1316_at 5.199478404
1320_at 4.956830441
1405_i_at 6.408205307
1431_at 11.55170858
1438_at 5.050453209
1487_at 7.242648757
1494_f_at 12.27148864
1598_g_at 7.45752086
160020_at 6.4223723
1729_at 6.501934008
177_at 4.950795716
1773_at 4.546015637
179_at 7.644670815
1861_at 5.247575449

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM449768_D-188.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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