|
| Status |
Public on Apr 18, 2023 |
| Title |
#1_shMfn2+Dox+TNF |
| Sample type |
SRA |
| |
|
| Source name |
endothelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human lung microvascular endothelial cells (HLMVECs)
lentivirus: shMfn2 RNA
treatment: doxycycline (200 ng/mL) + TNF
|
| Treatment protocol |
The cells were transduced with doxycycline inducible lentiviral shMfn2 RNA with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24h and then the media was changed. After 48h, the cells were treated with doxycycline (200 ng/mL) or DMSO (control) for 72h to knock- down endogenous Mfn2. For an exact biological control, we used lenti-Mfn2 shRNA expressing cells which were treated with DMSO and represented as Control. Mfn2-KD ECs were treated with doxycycline.
|
| Growth protocol |
Human lung microvascular endothelial cells (HLMVECs) were purchased from Lonza and cultured with EGM2 (Lonza) including all supplements and 10% FBS (Hyclone) until passage 8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by using phenol/chloroform and TRIzol Reagent, and its quality was evaluated with gel QC.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Gene expression data from human lung microvascular endothelial cells
|
| Data processing |
The sequenced reads from all samples were aligned to the human (hg38) reference genome with STAR v. 2.4.2, and the aligned reads were used to quantify mRNA expression by using HTSeq-count v. 0.6.1.
Genome_build: hg38
|
| |
|
| Submission date |
Apr 20, 2020 |
| Last update date |
Apr 18, 2023 |
| Contact name |
Jalees Rehman |
| E-mail(s) |
jalees@uic.edu
|
| Organization name |
UIC
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
909 S. Ashland Ave. MBRB 2072, Mailcode 669
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60607 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE148987 |
The Mitochondrial Protein Mitofusin 2 Stabilizes Adherens Junctions and Suppresses Endothelial Inflammation via Modualtion of β-catenin Signaling |
|
| Relations |
| BioSample |
SAMN14647175 |
| SRA |
SRX8144276 |
