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Sample GSM4483908 Query DataSets for GSM4483908
Status Public on May 31, 2020
Title LNM15
Sample type RNA
Source name LNM15
Organism Homo sapiens
Characteristics tissue: Lymph node metastasis
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
Hybridization protocol Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
Description microRNA expression of lymph node metastatic breast cancer.
Data processing The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
Submission date Apr 17, 2020
Last update date Jun 01, 2020
Contact name Nehad M Alajez
Organization name King Saud University College of Medicine
Department Anatomy
Lab Stem Cell Unit
Street address 1st floor College of Medicne
City Riyadh
ZIP/Postal code 11461
Country Saudi Arabia
Platform ID GPL21576
Series (2)
GSE148846 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients
GSE148848 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00015259 5.72082806
A_25_P00010378 -4.850988301
A_25_P00015257 3.861105908
A_25_P00015256 3.064188453
A_25_P00015255 7.076705797
A_25_P00015254 5.323377128
A_25_P00010373 2.088874083
A_25_P00010372 2.479024429
A_25_P00010371 3.606187294
A_25_P00010370 3.866424423
A_25_P00015781 9.466991916
A_25_P00015780 1.267139364
A_25_P00015783 -0.29315514
A_25_P00015782 4.320089196
A_25_P00015785 5.176754392
A_25_P00015784 1.419519343
A_25_P00015787 4.049795832
A_25_P00015786 4.463034273
A_25_P00015789 0.70721595
A_25_P00015788 3.386584281

Total number of rows: 5937

Table truncated, full table size 155 Kbytes.

Supplementary file Size Download File type/resource
GSM4483908_LNM15.txt.gz 2.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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