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Sample GSM4483899 Query DataSets for GSM4483899
Status Public on May 31, 2020
Title BC5
Sample type RNA
Source name BC5
Organism Homo sapiens
Characteristics tissue: Paired primary breast cancer
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
Hybridization protocol Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
Description microRNA expression of primary breast cancer.
Data processing The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
Submission date Apr 17, 2020
Last update date Jun 01, 2020
Contact name Nehad M Alajez
Organization name King Saud University College of Medicine
Department Anatomy
Lab Stem Cell Unit
Street address 1st floor College of Medicne
City Riyadh
ZIP/Postal code 11461
Country Saudi Arabia
Platform ID GPL21576
Series (2)
GSE148846 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients
GSE148848 microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00015259 7.252213844
A_25_P00010378 3.104971625
A_25_P00015257 3.975807603
A_25_P00015256 5.88282794
A_25_P00015255 7.711646868
A_25_P00015254 5.054917734
A_25_P00010373 2.698478964
A_25_P00010372 3.892997366
A_25_P00010371 3.424301866
A_25_P00010370 3.205453802
A_25_P00015781 2.230091018
A_25_P00015780 1.146857278
A_25_P00015783 3.579169527
A_25_P00015782 3.37017721
A_25_P00015785 2.164978243
A_25_P00015784 0.691481272
A_25_P00015787 3.774739973
A_25_P00015786 3.538066865
A_25_P00015789 2.098441395
A_25_P00015788 6.698861476

Total number of rows: 5937

Table truncated, full table size 155 Kbytes.

Supplementary file Size Download File type/resource
GSM4483899_BC5.txt.gz 2.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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