|
Status |
Public on May 31, 2020 |
Title |
BC8 |
Sample type |
RNA |
|
|
Source name |
BC8
|
Organism |
Homo sapiens |
Characteristics |
tissue: Paired primary breast cancer
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from FFPE sections using the recover all total nucleic acid isolation kit (Ambion Inc., Life Technologies, USA) according to the manufacturer’s protocol.
|
Label |
Cy3
|
Label protocol |
Cyanine-3 (Cy3) as per Agilent one-color (Agilent) labeling kit.
|
|
|
Hybridization protocol |
Labeled samples were hybridized to the Agilent 8 × 60k v16 miRNA microarray chip
|
Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting
|
Description |
microRNA expression of primary breast cancer.
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 11.0 (Agilent) using default parameters. Data were analyzed using Agilent GeneSpring X software
|
|
|
Submission date |
Apr 17, 2020 |
Last update date |
Jun 01, 2020 |
Contact name |
Nehad M Alajez |
E-mail(s) |
nalajez@gmail.com
|
Organization name |
King Saud University College of Medicine
|
Department |
Anatomy
|
Lab |
Stem Cell Unit
|
Street address |
1st floor College of Medicne
|
City |
Riyadh |
ZIP/Postal code |
11461 |
Country |
Saudi Arabia |
|
|
Platform ID |
GPL21576 |
Series (2) |
GSE148846 |
microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients |
GSE148848 |
microRNA expression profiling of paired primary and lymph node metastatic breast cancer patients, and gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors |
|